Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.)
In present study, EgSAPK (EU805512), an oil palm transcript coding for a putative SAPK protein kinase, have been molecular characterized. The cDNA for EgSAPK isolated from an oil palm cell suspension culture is 1470 bp in length with a longest Open Reading Frame (ORF) of 963 bp. No translation start...
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| Format: | Article |
| Language: | English English |
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Asian Network for Scientific Information
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16673/ http://psasir.upm.edu.my/id/eprint/16673/1/sequence%20and%20expression.docx |
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| author | Huynh, Ky Le Vinh, Thuc Siew-Eng, Ooi Ishak, Z. Namasivayam, Parameswari Napis, Suhaimi |
| author_facet | Huynh, Ky Le Vinh, Thuc Siew-Eng, Ooi Ishak, Z. Namasivayam, Parameswari Napis, Suhaimi |
| author_sort | Huynh, Ky |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In present study, EgSAPK (EU805512), an oil palm transcript coding for a putative SAPK protein kinase, have been molecular characterized. The cDNA for EgSAPK isolated from an oil palm cell suspension culture is 1470 bp in length with a longest Open Reading Frame (ORF) of 963 bp. No translation start codon could be identified so EgSAPK cDNA sequence is lacking the 5’-end. The deduced protein sequence shares 89% identity with the serine/threonine protein kinase SAPK9 from rice (AB125310.1). Real time PCR results showed that the expression levels of EgSAPK varied in different oil palm tissues and the EgSAPK gene shares a similar expression pattern with the SAPK gene of rice. Furthermore, the transcription of the EgSAPK gene in green embryo, white embryo and embryogenic calli tissues were higher than in non-embryogenic calli tissues. Southern blot analysis showed that the EgSAPK gene might be present as a single copy gene in the oil palm genome. These results suggest that EgSAPK may have a similar function as the SAPK gene of rice and thus can be a candidate marker for oil palm somatic embryogenesis. |
| first_indexed | 2025-11-15T08:08:29Z |
| format | Article |
| id | upm-16673 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:08:29Z |
| publishDate | 2009 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
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| spelling | upm-166732015-09-15T07:21:29Z http://psasir.upm.edu.my/id/eprint/16673/ Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) Huynh, Ky Le Vinh, Thuc Siew-Eng, Ooi Ishak, Z. Namasivayam, Parameswari Napis, Suhaimi In present study, EgSAPK (EU805512), an oil palm transcript coding for a putative SAPK protein kinase, have been molecular characterized. The cDNA for EgSAPK isolated from an oil palm cell suspension culture is 1470 bp in length with a longest Open Reading Frame (ORF) of 963 bp. No translation start codon could be identified so EgSAPK cDNA sequence is lacking the 5’-end. The deduced protein sequence shares 89% identity with the serine/threonine protein kinase SAPK9 from rice (AB125310.1). Real time PCR results showed that the expression levels of EgSAPK varied in different oil palm tissues and the EgSAPK gene shares a similar expression pattern with the SAPK gene of rice. Furthermore, the transcription of the EgSAPK gene in green embryo, white embryo and embryogenic calli tissues were higher than in non-embryogenic calli tissues. Southern blot analysis showed that the EgSAPK gene might be present as a single copy gene in the oil palm genome. These results suggest that EgSAPK may have a similar function as the SAPK gene of rice and thus can be a candidate marker for oil palm somatic embryogenesis. Asian Network for Scientific Information 2009 Article PeerReviewed application/msword en http://psasir.upm.edu.my/id/eprint/16673/1/sequence%20and%20expression.docx Huynh, Ky and Le Vinh, Thuc and Siew-Eng, Ooi and Ishak, Z. and Namasivayam, Parameswari and Napis, Suhaimi (2009) Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.). International Journal of Botany, 5 (1). pp. 76-84. ISSN 1811-9700 English |
| spellingShingle | Huynh, Ky Le Vinh, Thuc Siew-Eng, Ooi Ishak, Z. Namasivayam, Parameswari Napis, Suhaimi Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title | Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title_full | Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title_fullStr | Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title_full_unstemmed | Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title_short | Sequence and expression analysis of EgSAPK, a putative member of the serine/threonine protein kinases in oil palm (Elaeis guineensis Jacq.) |
| title_sort | sequence and expression analysis of egsapk, a putative member of the serine/threonine protein kinases in oil palm (elaeis guineensis jacq.) |
| url | http://psasir.upm.edu.my/id/eprint/16673/ http://psasir.upm.edu.my/id/eprint/16673/1/sequence%20and%20expression.docx |