Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl
The vacuolar-type H+-ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated fr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Informa UK
2008
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16672/ http://psasir.upm.edu.my/id/eprint/16672/1/16672.pdf |
| _version_ | 1848843026710921216 |
|---|---|
| author | Ho, Chai Ling Nguyen, Phuoc Dang Harikrishna, Jennifer Ann Abdul Rahim, Raha |
| author_facet | Ho, Chai Ling Nguyen, Phuoc Dang Harikrishna, Jennifer Ann Abdul Rahim, Raha |
| author_sort | Ho, Chai Ling |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The vacuolar-type H+-ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated from Acanthus ebracteatus, and selected for full-length sequencing. The 876-nucleotide cDNA consists of an open reading frame of 165 amino acids. The deduced amino acid sequence displays high similarity (81%) with its homologs from Arabidopsis thaliana, Avecinnia marina and Gossypium hirsutum with the four transmembrane domains characteristics of the 16 kDa proteolipid subunit c of V-ATPase well conserved in this protein. Southern analysis revealed the existence of several members of proteolipid subunit c of V-ATPase in A. ebracteatus. The mRNA of this gene was detected in leaf, floral, stem and root tissues, however, the expression level was lower in stem and root tissues. |
| first_indexed | 2025-11-15T08:08:29Z |
| format | Article |
| id | upm-16672 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:08:29Z |
| publishDate | 2008 |
| publisher | Informa UK |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-166722019-10-22T02:20:32Z http://psasir.upm.edu.my/id/eprint/16672/ Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl Ho, Chai Ling Nguyen, Phuoc Dang Harikrishna, Jennifer Ann Abdul Rahim, Raha The vacuolar-type H+-ATPase (V-ATPase) is a multimeric enzyme with diverse functions in plants such as nutrient transport, flowering, stress tolerance, guard cell movement and development. A partial sequence of V-ATPase proteolipid was identified among the expressed sequence tags (ESTs) generated from Acanthus ebracteatus, and selected for full-length sequencing. The 876-nucleotide cDNA consists of an open reading frame of 165 amino acids. The deduced amino acid sequence displays high similarity (81%) with its homologs from Arabidopsis thaliana, Avecinnia marina and Gossypium hirsutum with the four transmembrane domains characteristics of the 16 kDa proteolipid subunit c of V-ATPase well conserved in this protein. Southern analysis revealed the existence of several members of proteolipid subunit c of V-ATPase in A. ebracteatus. The mRNA of this gene was detected in leaf, floral, stem and root tissues, however, the expression level was lower in stem and root tissues. Informa UK 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/16672/1/16672.pdf Ho, Chai Ling and Nguyen, Phuoc Dang and Harikrishna, Jennifer Ann and Abdul Rahim, Raha (2008) Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl. DNA Sequence, 19 (1). pp. 73-77. ISSN 1042-5179; ESSN: 1029-2365 https://www.tandfonline.com/doi/abs/10.1080/10425170701445501 10.1080/10425170701445501 |
| spellingShingle | Ho, Chai Ling Nguyen, Phuoc Dang Harikrishna, Jennifer Ann Abdul Rahim, Raha Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title | Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title_full | Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title_fullStr | Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title_full_unstemmed | Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title_short | Sequence analysis and characterization of vacuolar-type H+-ATPase proteolipid transcript from Acanthus ebracteatus Vahl |
| title_sort | sequence analysis and characterization of vacuolar-type h+-atpase proteolipid transcript from acanthus ebracteatus vahl |
| url | http://psasir.upm.edu.my/id/eprint/16672/ http://psasir.upm.edu.my/id/eprint/16672/ http://psasir.upm.edu.my/id/eprint/16672/ http://psasir.upm.edu.my/id/eprint/16672/1/16672.pdf |