Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.)
Problem statement: The effect of cytokinins on scalp induction from in vitro propagating shoot tips of different banana (Musa spp. AAA and AAB) cultivars was studied. Approach: Proliferation rate increased with an increasing concentration of Benzyl Amino Purine(BAP) up to 22.2 μM while, lower level...
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| Format: | Article |
| Language: | English |
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Science Publications
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16625/ http://psasir.upm.edu.my/id/eprint/16625/1/ajabssp.2010.128.134.pdf |
| _version_ | 1848843013764153344 |
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| author | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah |
| author_facet | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah |
| author_sort | Bidabadi, Siamak Shirani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Problem statement: The effect of cytokinins on scalp induction from in vitro propagating shoot tips of different banana (Musa spp. AAA and AAB) cultivars was studied.
Approach: Proliferation rate increased with an increasing concentration of Benzyl Amino Purine(BAP) up to 22.2 μM while, lower level of BAP (11.1 μM) increased scalp induction. Results: Kinetin induced a lower number of shoots than that obtained with similar levels of BAP and caused an increase in scalp induction rate at higher concentration (22.2 μM). Shoot proliferation in ‘Rastali’(AAB),‘Berangan Intan’ and ‘Berangan’(AAA) significantly increased with increasing Thidiazuron (TDZ)
concentration from 3.17, 2.17 and 3.33 shoots at 0.5 μM to 14.17, 6.22 and 6.17 shoots at 5 μM respectively, as concentrations above 5 μM for all cultivars were inhibitor.
Conclusion/Recommendations: The highest ratio of scalp formation (8.89) was recorded in ‘Rastali’ at the highest concentration of TDZ (7.5 μM), but in ‘Berangan Intan’ and ‘Berangan’ (AAA) TDZ increased scalp induction rate from 0.00 and 0.43 at 0.5 μM to 4.22 and 2.67 at 5 μM respectively before falling to 2.11 for both at 7.5 μM. BAP at 22.2 μM was considered optimal for shoot proliferation as well as shoot elongation from excised scalps of banana cultivars. |
| first_indexed | 2025-11-15T08:08:17Z |
| format | Article |
| id | upm-16625 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:08:17Z |
| publishDate | 2010 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-166252017-11-21T05:39:10Z http://psasir.upm.edu.my/id/eprint/16625/ Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah Problem statement: The effect of cytokinins on scalp induction from in vitro propagating shoot tips of different banana (Musa spp. AAA and AAB) cultivars was studied. Approach: Proliferation rate increased with an increasing concentration of Benzyl Amino Purine(BAP) up to 22.2 μM while, lower level of BAP (11.1 μM) increased scalp induction. Results: Kinetin induced a lower number of shoots than that obtained with similar levels of BAP and caused an increase in scalp induction rate at higher concentration (22.2 μM). Shoot proliferation in ‘Rastali’(AAB),‘Berangan Intan’ and ‘Berangan’(AAA) significantly increased with increasing Thidiazuron (TDZ) concentration from 3.17, 2.17 and 3.33 shoots at 0.5 μM to 14.17, 6.22 and 6.17 shoots at 5 μM respectively, as concentrations above 5 μM for all cultivars were inhibitor. Conclusion/Recommendations: The highest ratio of scalp formation (8.89) was recorded in ‘Rastali’ at the highest concentration of TDZ (7.5 μM), but in ‘Berangan Intan’ and ‘Berangan’ (AAA) TDZ increased scalp induction rate from 0.00 and 0.43 at 0.5 μM to 4.22 and 2.67 at 5 μM respectively before falling to 2.11 for both at 7.5 μM. BAP at 22.2 μM was considered optimal for shoot proliferation as well as shoot elongation from excised scalps of banana cultivars. Science Publications 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16625/1/ajabssp.2010.128.134.pdf Bidabadi, Siamak Shirani and Meon, Sariah and Wahab, Zakaria and Mahmood, Maziah (2010) Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.). American Journal of Agricultural and Biological Sciences, 5 (2). pp. 128-134. ISSN 1557-4989; ESSN: 1557-4997 http://thescipub.com/html/10.3844/ajabssp.2010.128.134 10.3844/ajabssp.2010.128.134 |
| spellingShingle | Bidabadi, Siamak Shirani Meon, Sariah Wahab, Zakaria Mahmood, Maziah Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title | Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title_full | Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title_fullStr | Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title_full_unstemmed | Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title_short | Scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (Musa spp.) |
| title_sort | scalp induction rate responses to cytokinins on proliferating shoot-tips of banana cultivars (musa spp.) |
| url | http://psasir.upm.edu.my/id/eprint/16625/ http://psasir.upm.edu.my/id/eprint/16625/ http://psasir.upm.edu.my/id/eprint/16625/ http://psasir.upm.edu.my/id/eprint/16625/1/ajabssp.2010.128.134.pdf |