RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene
The potential health benefits of carotenoids as anti cancer and antioxidant agents have recently been demonstrated. Oil palm, Elaeis oleifera in particular, is known to be the richest natural source for carotene. However, the species has not been commercially exploited due to its extremely low oil y...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16604/ http://psasir.upm.edu.my/id/eprint/16604/1/RT-PCR%20amplification%20and%20cloning%20of%20partial%20DNA%20sequence%20coding%20for%20oil%20palm%20%28Elaeis%20oleifera%29%20phytoene%20synthase%20gene.pdf |
| _version_ | 1848843007989645312 |
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| author | Abd Rasid, Omar Wan Sulaiman, Wan Nurul Syuhada Ayub, Nor Hanin Subhi, Siti Masura Mohamed, Zulqarnain Ho, Chai Ling Sambanthamurthi, Ravigadevi Napis, Suhaimi |
| author_facet | Abd Rasid, Omar Wan Sulaiman, Wan Nurul Syuhada Ayub, Nor Hanin Subhi, Siti Masura Mohamed, Zulqarnain Ho, Chai Ling Sambanthamurthi, Ravigadevi Napis, Suhaimi |
| author_sort | Abd Rasid, Omar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The potential health benefits of carotenoids as anti cancer and antioxidant agents have recently been demonstrated. Oil palm, Elaeis oleifera in particular, is known to be the richest natural source for carotene. However, the species has not been commercially exploited due to its extremely low oil yield. The current work describes the isolation of a cDNA clone coding for phytoene synthase (psy) from E. oleifera by RT-PCR amplification. A pair of psy gene specific primers was successfully used to amplify a 899 bp fragment that codes for a partial length (300 amino acids) of oil palm psy. The DNA and amino acid sequences were shown to share a high level of identity to phytoene synthase from other plants at about 83%. Further analysis also showed the presence of conserved aspartate-rich catalytic domains within the clone. Work was also carried out to obtain the expression pattern of oil palm psy in developing fruits by real-time PCR analysis. Results indicated that the gene is highly regulated during the course of oil palm fruit development. The pattern of psy expression was shown to be well correlated to the accumulation of lutein in the young mesocarp and α- and β-carotenes in the older tissues. This observation demonstrated that oil palm psy was highly regulated for tissue development and accumulation of carotenes for storage. |
| first_indexed | 2025-11-15T08:08:11Z |
| format | Article |
| id | upm-16604 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:08:11Z |
| publishDate | 2008 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-166042018-01-22T06:32:45Z http://psasir.upm.edu.my/id/eprint/16604/ RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene Abd Rasid, Omar Wan Sulaiman, Wan Nurul Syuhada Ayub, Nor Hanin Subhi, Siti Masura Mohamed, Zulqarnain Ho, Chai Ling Sambanthamurthi, Ravigadevi Napis, Suhaimi The potential health benefits of carotenoids as anti cancer and antioxidant agents have recently been demonstrated. Oil palm, Elaeis oleifera in particular, is known to be the richest natural source for carotene. However, the species has not been commercially exploited due to its extremely low oil yield. The current work describes the isolation of a cDNA clone coding for phytoene synthase (psy) from E. oleifera by RT-PCR amplification. A pair of psy gene specific primers was successfully used to amplify a 899 bp fragment that codes for a partial length (300 amino acids) of oil palm psy. The DNA and amino acid sequences were shown to share a high level of identity to phytoene synthase from other plants at about 83%. Further analysis also showed the presence of conserved aspartate-rich catalytic domains within the clone. Work was also carried out to obtain the expression pattern of oil palm psy in developing fruits by real-time PCR analysis. Results indicated that the gene is highly regulated during the course of oil palm fruit development. The pattern of psy expression was shown to be well correlated to the accumulation of lutein in the young mesocarp and α- and β-carotenes in the older tissues. This observation demonstrated that oil palm psy was highly regulated for tissue development and accumulation of carotenes for storage. Malaysian Society for Molecular Biology and Biotechnology 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16604/1/RT-PCR%20amplification%20and%20cloning%20of%20partial%20DNA%20sequence%20coding%20for%20oil%20palm%20%28Elaeis%20oleifera%29%20phytoene%20synthase%20gene.pdf Abd Rasid, Omar and Wan Sulaiman, Wan Nurul Syuhada and Ayub, Nor Hanin and Subhi, Siti Masura and Mohamed, Zulqarnain and Ho, Chai Ling and Sambanthamurthi, Ravigadevi and Napis, Suhaimi (2008) RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene. Asia Pacific Journal of Molecular Biology and Biotechnology, 16 (1). pp. 17-24. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html161/161c.htm |
| spellingShingle | Abd Rasid, Omar Wan Sulaiman, Wan Nurul Syuhada Ayub, Nor Hanin Subhi, Siti Masura Mohamed, Zulqarnain Ho, Chai Ling Sambanthamurthi, Ravigadevi Napis, Suhaimi RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title | RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title_full | RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title_fullStr | RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title_full_unstemmed | RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title_short | RT-PCR amplification and cloning of partial DNA sequence coding for oil palm (Elaeis oleifera) phytoene synthase gene |
| title_sort | rt-pcr amplification and cloning of partial dna sequence coding for oil palm (elaeis oleifera) phytoene synthase gene |
| url | http://psasir.upm.edu.my/id/eprint/16604/ http://psasir.upm.edu.my/id/eprint/16604/ http://psasir.upm.edu.my/id/eprint/16604/1/RT-PCR%20amplification%20and%20cloning%20of%20partial%20DNA%20sequence%20coding%20for%20oil%20palm%20%28Elaeis%20oleifera%29%20phytoene%20synthase%20gene.pdf |