Rhaphidophora decursiva leaves: phenolic content and antioxidant activity

Total phenolic content (TPC) and antioxidant activity (AA) of Rhaphidophora decursiva (Roxb.) Schott leaves extracted with methanol and water were studied. The TPC was assessed using Folin-Ciocalteu method. Meanwhile, the antioxidant activity was estimated using β-carotene bleaching, 2, 2-diphenyl-2...

Full description

Bibliographic Details
Main Authors: Mohd Esa, Norhaizan, Phuah, See Cheng
Format: Article
Language:English
Published: Malaysian Agricultural Research and Development Institute (MARDI) 2009
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/16572/
http://psasir.upm.edu.my/id/eprint/16572/1/Rhaphidophora%20decursiva%20leaves.pdf
_version_ 1848842998943580160
author Mohd Esa, Norhaizan
Phuah, See Cheng
author_facet Mohd Esa, Norhaizan
Phuah, See Cheng
author_sort Mohd Esa, Norhaizan
building UPM Institutional Repository
collection Online Access
description Total phenolic content (TPC) and antioxidant activity (AA) of Rhaphidophora decursiva (Roxb.) Schott leaves extracted with methanol and water were studied. The TPC was assessed using Folin-Ciocalteu method. Meanwhile, the antioxidant activity was estimated using β-carotene bleaching, 2, 2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, and ferric reducing antioxidant power (FRAP) assay. Butylated hydroxytoluene (BHT) was used to compare its AA with the other extracts. It was found that the total AA of methanol extracts was higher than that of BHT. Significant differences were found in the scavenging activity, EC50 value and TPC between the water and methanol extracts. No significant relationship (p>0.05) between TPC and AA as measured by β-carotene bleaching and DPPH radical scavenging methods for both water and methanol extracts. However, TPC and AA as measured by FRAP assay did show a significant relationship at p<0.01 level. This study showed that the methanol extracts of the plant possess both higher TPC and AA compared to that of water extracts, and this was supported by the consistent results showed by all antioxidant activity assays. The antioxidant properties of the plant may have originated not only from phenolic but also from the non-phenolic compounds which possess high antioxidant activity.
first_indexed 2025-11-15T08:08:03Z
format Article
id upm-16572
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T08:08:03Z
publishDate 2009
publisher Malaysian Agricultural Research and Development Institute (MARDI)
recordtype eprints
repository_type Digital Repository
spelling upm-165722016-10-10T04:46:20Z http://psasir.upm.edu.my/id/eprint/16572/ Rhaphidophora decursiva leaves: phenolic content and antioxidant activity Mohd Esa, Norhaizan Phuah, See Cheng Total phenolic content (TPC) and antioxidant activity (AA) of Rhaphidophora decursiva (Roxb.) Schott leaves extracted with methanol and water were studied. The TPC was assessed using Folin-Ciocalteu method. Meanwhile, the antioxidant activity was estimated using β-carotene bleaching, 2, 2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, and ferric reducing antioxidant power (FRAP) assay. Butylated hydroxytoluene (BHT) was used to compare its AA with the other extracts. It was found that the total AA of methanol extracts was higher than that of BHT. Significant differences were found in the scavenging activity, EC50 value and TPC between the water and methanol extracts. No significant relationship (p>0.05) between TPC and AA as measured by β-carotene bleaching and DPPH radical scavenging methods for both water and methanol extracts. However, TPC and AA as measured by FRAP assay did show a significant relationship at p<0.01 level. This study showed that the methanol extracts of the plant possess both higher TPC and AA compared to that of water extracts, and this was supported by the consistent results showed by all antioxidant activity assays. The antioxidant properties of the plant may have originated not only from phenolic but also from the non-phenolic compounds which possess high antioxidant activity. Malaysian Agricultural Research and Development Institute (MARDI) 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16572/1/Rhaphidophora%20decursiva%20leaves.pdf Mohd Esa, Norhaizan and Phuah, See Cheng (2009) Rhaphidophora decursiva leaves: phenolic content and antioxidant activity. Journal of Tropical Agriculture and Food Science, 37 (1). pp. 61-66. ISSN 1394-9829; ESSN: 2289-9650 http://ejtafs.mardi.gov.my/index.php/publication/issues/archive/35-2009/volume-37-no1/77-370108 Medicinal plants. Materia medica, Vegetable. Herbs - Therapeutic use.
spellingShingle Medicinal plants.
Materia medica, Vegetable.
Herbs - Therapeutic use.
Mohd Esa, Norhaizan
Phuah, See Cheng
Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title_full Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title_fullStr Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title_full_unstemmed Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title_short Rhaphidophora decursiva leaves: phenolic content and antioxidant activity
title_sort rhaphidophora decursiva leaves: phenolic content and antioxidant activity
topic Medicinal plants.
Materia medica, Vegetable.
Herbs - Therapeutic use.
url http://psasir.upm.edu.my/id/eprint/16572/
http://psasir.upm.edu.my/id/eprint/16572/
http://psasir.upm.edu.my/id/eprint/16572/1/Rhaphidophora%20decursiva%20leaves.pdf