Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor
A dual response approach using diacylglycerol (DAG) and triacylglycerol (TAG) as responses for optimization of 1-stearoyl-3(2)-oleoyl glycerol-enriched DAG synthesis using response surface methodology (RSM) was investigated. Four variables from a lipase-catalyzed esterification reaction were optimiz...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16538/ http://psasir.upm.edu.my/id/eprint/16538/1/Response%20surface%20modelling%20of%201.pdf |
| _version_ | 1848842990126104576 |
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| author | Lo, Seong Koon Arifin, Norlelawati Cheong, Ling Zhi Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_facet | Lo, Seong Koon Arifin, Norlelawati Cheong, Ling Zhi Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming |
| author_sort | Lo, Seong Koon |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A dual response approach using diacylglycerol (DAG) and triacylglycerol (TAG) as responses for optimization of 1-stearoyl-3(2)-oleoyl glycerol-enriched DAG synthesis using response surface methodology (RSM) was investigated. Four variables from a lipase-catalyzed esterification reaction were optimized using a central composite rotatable design. The following optimized conditions yielded 51 wt.% DAG and 22 wt.% TAG: reaction temperature of 55 °C, enzyme dosage of 9.5 wt.%, fatty acid/glycerol molar ratio of 2.1 and reaction time of 3 h. Results were repeatable at 10 kg production scale in a pilot packed-bed enzyme reactor. No significant losses in enzyme activity or changes in fatty acid selectivity on DAG synthesis were observed during the five pilot productions. Lipozyme RM IM showed selectivity towards the production of stearic acid enriched DAG. The purity of DAG oil after purification was 90 wt.%. |
| first_indexed | 2025-11-15T08:07:54Z |
| format | Article |
| id | upm-16538 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:54Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-165382018-04-26T04:08:51Z http://psasir.upm.edu.my/id/eprint/16538/ Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor Lo, Seong Koon Arifin, Norlelawati Cheong, Ling Zhi Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming A dual response approach using diacylglycerol (DAG) and triacylglycerol (TAG) as responses for optimization of 1-stearoyl-3(2)-oleoyl glycerol-enriched DAG synthesis using response surface methodology (RSM) was investigated. Four variables from a lipase-catalyzed esterification reaction were optimized using a central composite rotatable design. The following optimized conditions yielded 51 wt.% DAG and 22 wt.% TAG: reaction temperature of 55 °C, enzyme dosage of 9.5 wt.%, fatty acid/glycerol molar ratio of 2.1 and reaction time of 3 h. Results were repeatable at 10 kg production scale in a pilot packed-bed enzyme reactor. No significant losses in enzyme activity or changes in fatty acid selectivity on DAG synthesis were observed during the five pilot productions. Lipozyme RM IM showed selectivity towards the production of stearic acid enriched DAG. The purity of DAG oil after purification was 90 wt.%. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16538/1/Response%20surface%20modelling%20of%201.pdf Lo, Seong Koon and Arifin, Norlelawati and Cheong, Ling Zhi and Tan, Chin Ping and Long, Kamariah and Yusoff, Mohd Suria Affandi and Lai, Oi Ming (2009) Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor. Journal of Molecular Catalysis B: Enzymatic, 57 (1-4). pp. 136-144. ISSN 1381-1177; ESSN: 1873-3158 10.1016/j.molcatb.2008.08.010 |
| spellingShingle | Lo, Seong Koon Arifin, Norlelawati Cheong, Ling Zhi Tan, Chin Ping Long, Kamariah Yusoff, Mohd Suria Affandi Lai, Oi Ming Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title | Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title_full | Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title_fullStr | Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title_full_unstemmed | Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title_short | Response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized Rhizomucor miehei lipase reactor |
| title_sort | response surface modelling of 1-stearoyl-3(2)-oleoly glycerol production in a pilot packed bed immobilized rhizomucor miehei lipase reactor |
| url | http://psasir.upm.edu.my/id/eprint/16538/ http://psasir.upm.edu.my/id/eprint/16538/ http://psasir.upm.edu.my/id/eprint/16538/1/Response%20surface%20modelling%20of%201.pdf |