Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells
Background and Aim: Nigella sativa and its active constituent thymoquinone (TQ) have been exploited for their various health benefits. This work was aimed to investigate the regulatory effects of TQ-rich fraction (TQRF) and commercial TQ on the low-density lipoprotein receptor (LDLR) and 3-hydroxy-3...
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| Format: | Article |
| Language: | English |
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Karger
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16479/ http://psasir.upm.edu.my/id/eprint/16479/1/Regulation%20of%20low.pdf |
| _version_ | 1848842974082891776 |
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| author | Al-Naqeep, Ghanya Ismail, Maznah Allaudin, Zeenathul Nazariah |
| author_facet | Al-Naqeep, Ghanya Ismail, Maznah Allaudin, Zeenathul Nazariah |
| author_sort | Al-Naqeep, Ghanya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background and Aim: Nigella sativa and its active constituent thymoquinone (TQ) have been exploited for their various health benefits. This work was aimed to investigate the regulatory effects of TQ-rich fraction (TQRF) and commercial TQ on the low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) genes in HepG2 cells. Methods and Results: TQRF was extracted from N. sativa seeds using supercritical fluid extraction. The regulatory effects of TQRF at 80 μg/ml and TQ at 2 μg/ml on LDLR and HMGCR gene expression were investigated in HepG2 cells using quantitative real-time PCR. The TQ content in TQRF was 2.77% (w/w) and was obtained at a temperature of 40°C and a pressure of 600 bar. Treatment of cells with TQRF and TQ resulted in a 7- and 2-fold upregulation of LDLR mRNA level, respectively, compared with untreated cells. The mRNA level of HMGCR was downregulated by 71 and 12%, respectively, compared with untreated cells. Conclusion: TQRF and TQ regulated genes involved in cholesterol metabolism by two mechanisms, the uptake of low-density lipoprotein cholesterol via the upregulation of the LDLR gene and inhibition of cholesterol synthesis via the suppression of the HMGCR gene. |
| first_indexed | 2025-11-15T08:07:39Z |
| format | Article |
| id | upm-16479 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:39Z |
| publishDate | 2009 |
| publisher | Karger |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164792019-10-30T08:51:24Z http://psasir.upm.edu.my/id/eprint/16479/ Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells Al-Naqeep, Ghanya Ismail, Maznah Allaudin, Zeenathul Nazariah Background and Aim: Nigella sativa and its active constituent thymoquinone (TQ) have been exploited for their various health benefits. This work was aimed to investigate the regulatory effects of TQ-rich fraction (TQRF) and commercial TQ on the low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) genes in HepG2 cells. Methods and Results: TQRF was extracted from N. sativa seeds using supercritical fluid extraction. The regulatory effects of TQRF at 80 μg/ml and TQ at 2 μg/ml on LDLR and HMGCR gene expression were investigated in HepG2 cells using quantitative real-time PCR. The TQ content in TQRF was 2.77% (w/w) and was obtained at a temperature of 40°C and a pressure of 600 bar. Treatment of cells with TQRF and TQ resulted in a 7- and 2-fold upregulation of LDLR mRNA level, respectively, compared with untreated cells. The mRNA level of HMGCR was downregulated by 71 and 12%, respectively, compared with untreated cells. Conclusion: TQRF and TQ regulated genes involved in cholesterol metabolism by two mechanisms, the uptake of low-density lipoprotein cholesterol via the upregulation of the LDLR gene and inhibition of cholesterol synthesis via the suppression of the HMGCR gene. Karger 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16479/1/Regulation%20of%20low.pdf Al-Naqeep, Ghanya and Ismail, Maznah and Allaudin, Zeenathul Nazariah (2009) Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells. Journal of Nutrigenetics and Nutrigenomics, 2 (4-5). pp. 163-172. ISSN 1661-6499; ESSN: 1661-6758 https://www.karger.com/Article/Abstract/227264 10.1159/000227264 |
| spellingShingle | Al-Naqeep, Ghanya Ismail, Maznah Allaudin, Zeenathul Nazariah Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title | Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title_full | Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title_fullStr | Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title_full_unstemmed | Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title_short | Regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase gene expression by thymoquinone-rich fraction and thymoquinone in HepG2 cells |
| title_sort | regulation of low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme a reductase gene expression by thymoquinone-rich fraction and thymoquinone in hepg2 cells |
| url | http://psasir.upm.edu.my/id/eprint/16479/ http://psasir.upm.edu.my/id/eprint/16479/ http://psasir.upm.edu.my/id/eprint/16479/ http://psasir.upm.edu.my/id/eprint/16479/1/Regulation%20of%20low.pdf |