Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells.
Thymoquinone (TQ) rich fraction (TQRF) extracted from Nigella sativa seeds using a supercritical fluid extraction technique was prepared. The regulatory effects of TQRF at 80 µg/mL and commercial TQ at 2 µg/mL on apolipoprotein B100 (Apo B100) and apolipoprotein A-1 (Apo A-1) genes in the presence o...
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| Format: | Article |
| Language: | English |
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Wiley-Blackwell
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16478/ http://psasir.upm.edu.my/id/eprint/16478/1/Regulation%20of%20Apolipoprotein%20A.pdf |
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| author | Al Naqeeb, Ghanya Ismail, Maznah |
| author_facet | Al Naqeeb, Ghanya Ismail, Maznah |
| author_sort | Al Naqeeb, Ghanya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thymoquinone (TQ) rich fraction (TQRF) extracted from Nigella sativa seeds using a supercritical fluid extraction technique was prepared. The regulatory effects of TQRF at 80 µg/mL and commercial TQ at 2 µg/mL on apolipoprotein B100 (Apo B100) and apolipoprotein A-1 (Apo A-1) genes in the presence or absence of 25-hydroxycholesterol (25OH), were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. Incubating HepG2 cells in 10% human lipoprotein deficient serum (HLPDS) for 24 h in the presence of 2 µg/mL 25OH showed a significant increase in Apo B100 mRNA expression level by twofold compared to the control cells; on the other hand, no significant change in Apo A-1 mRNA level was observed. When cells were incubated with HLPDS in the absence of 25OH and treated with TQRF and TQ, the mRNA level of Apo B100 was down-regulated by 70 and 49%, respectively, in TQRF and TQ treated cells compared to untreated cells. Apo A-1 gene was up-regulated by four- and twofold in TQRF and TQ treated cells, respectively, compared to that observed in untreated cells. The present study clearly shows that TQRF and TQ are effective in regulating Apo A-1 and Apo B100 genes that influence cholesterol metabolism in HepG2 cells.
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| first_indexed | 2025-11-15T08:07:39Z |
| format | Article |
| id | upm-16478 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:39Z |
| publishDate | 2009 |
| publisher | Wiley-Blackwell |
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| spelling | upm-164782015-11-16T03:16:19Z http://psasir.upm.edu.my/id/eprint/16478/ Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. Al Naqeeb, Ghanya Ismail, Maznah Thymoquinone (TQ) rich fraction (TQRF) extracted from Nigella sativa seeds using a supercritical fluid extraction technique was prepared. The regulatory effects of TQRF at 80 µg/mL and commercial TQ at 2 µg/mL on apolipoprotein B100 (Apo B100) and apolipoprotein A-1 (Apo A-1) genes in the presence or absence of 25-hydroxycholesterol (25OH), were investigated in human HepG2 cell line using quantitative real-time polymerase chain reaction. Incubating HepG2 cells in 10% human lipoprotein deficient serum (HLPDS) for 24 h in the presence of 2 µg/mL 25OH showed a significant increase in Apo B100 mRNA expression level by twofold compared to the control cells; on the other hand, no significant change in Apo A-1 mRNA level was observed. When cells were incubated with HLPDS in the absence of 25OH and treated with TQRF and TQ, the mRNA level of Apo B100 was down-regulated by 70 and 49%, respectively, in TQRF and TQ treated cells compared to untreated cells. Apo A-1 gene was up-regulated by four- and twofold in TQRF and TQ treated cells, respectively, compared to that observed in untreated cells. The present study clearly shows that TQRF and TQ are effective in regulating Apo A-1 and Apo B100 genes that influence cholesterol metabolism in HepG2 cells. Wiley-Blackwell 2009-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16478/1/Regulation%20of%20Apolipoprotein%20A.pdf Al Naqeeb, Ghanya and Ismail, Maznah (2009) Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. Journal of Food Lipids, 16 (2). pp. 245-258. ISSN 1065-7258 http://as.wiley.com/WileyCDA/Brand/id-35.html Black cumin - Research Apolipoproteins Black cumin - Therapeutic use 10.1111/j.1745-4522.2009.01144.x |
| spellingShingle | Black cumin - Research Apolipoproteins Black cumin - Therapeutic use Al Naqeeb, Ghanya Ismail, Maznah Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title | Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title_full | Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title_fullStr | Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title_full_unstemmed | Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title_short | Regulation of Apolipoprotein A-1 and Apolipoprotein B100 Genes by Thymoquinone Rich Fraction and Thymoquinone in HEPG2 Cells. |
| title_sort | regulation of apolipoprotein a-1 and apolipoprotein b100 genes by thymoquinone rich fraction and thymoquinone in hepg2 cells. |
| topic | Black cumin - Research Apolipoproteins Black cumin - Therapeutic use |
| url | http://psasir.upm.edu.my/id/eprint/16478/ http://psasir.upm.edu.my/id/eprint/16478/ http://psasir.upm.edu.my/id/eprint/16478/ http://psasir.upm.edu.my/id/eprint/16478/1/Regulation%20of%20Apolipoprotein%20A.pdf |