Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantificatio...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16440/ http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf |
| _version_ | 1848842963343376384 |
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| author | Kesar Singh, Jasbeer Kaur Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen |
| author_facet | Kesar Singh, Jasbeer Kaur Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen |
| author_sort | Kesar Singh, Jasbeer Kaur |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia. |
| first_indexed | 2025-11-15T08:07:29Z |
| format | Article |
| id | upm-16440 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:29Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164402015-09-27T23:40:06Z http://psasir.upm.edu.my/id/eprint/16440/ Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia Kesar Singh, Jasbeer Kaur Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia. Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf Kesar Singh, Jasbeer Kaur and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen (2010) Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia. Food Control, 21 (11). pp. 1536-1544. ISSN 0956-7135; ESSN: 1873-7129 10.1016/j.foodcont.2010.03.018 |
| spellingShingle | Kesar Singh, Jasbeer Kaur Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title | Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title_full | Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title_fullStr | Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title_full_unstemmed | Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title_short | Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
| title_sort | real-time pcr-based detection and quantification of genetically modified maize in processed feeds commercialised in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/16440/ http://psasir.upm.edu.my/id/eprint/16440/ http://psasir.upm.edu.my/id/eprint/16440/1/Real.pdf |