Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis

QR Code

Real-time PCR evaluation of seven DNA extraction methods for the purpose of GMO analysis

Successful DNA amplification is vital for the detection of specific DNA targets in feeds, and this in return depends on the ability of DNA extraction methods to produce good quality DNA. In this study, seven methods were compared for DNA extraction from feeds using quantitative polymerase chain re...

Full description

Bibliographic Details
Main Authors:	Kesar Singh, Jasbeer Kaur, Radu, Son, Mohamad Ghazali, Farinazleen, Cheah, Yoke Kqueen
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2009
Online Access:	http://psasir.upm.edu.my/id/eprint/16439/ http://psasir.upm.edu.my/id/eprint/16439/1/16439.pdf

Similar Items

Application of DNA and immunoassay analytical methods for GMO testing in agricultural crops and plant-derived products
by: Kesar Singh, Jasbeer Kaur, et al.
Published: (2008)

Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia
by: Kesar Singh, Jasbeer Kaur, et al.
Published: (2010)

Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples
by: Cheah, Yoke Kqueen, et al.
Published: (2011)

Quantification of Genetically Modified Maize in Animal Feed Using Real-Time Polymerase Chain Reaction
by: Kesar Singh, Jasbeer Kaur
Published: (2011)

Quantitative analysis of Roundup Ready soybean content in soy-derived food and animal feed by using Real-time PCR incorporated with cloned DNA fragments
by: Khoo, Siew Ping, et al.
Published: (2011)

The used of recombinant plasmid DNA in GMO quantitative analysis of insect resistance maize targeted unapproved StarLink corn and approved Bt176 corn in food and feed sold commercially sold Malaysia
by: Khoo, Siew Ping, et al.
Published: (2011)

Surface plasmon resonance biosensor for real-time detection of genetically modified organisms
by: Cheah, Yoke Kqueen, et al.
Published: (2010)

Quantitative Real-Time PCR in aDNA Research
by: Bunce, Michael, et al.
Published: (2012)

Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR
by: Ponniah, Jeyaletchumi, et al.
Published: (2010)

DNA extraction and species detection in gelatin and gelatin capsule by real-time PCR
by: Mohamad, Nurhidayatul Asma, et al.
Published: (2014)

Ethical evaluation of GMO from an Islamic perspective
by: Mahmod, Safa Senan, et al.
Published: (2013)

Meatball model of porcine DNA detection by TaqMan probe real-time PCR
by: Sajali, N., et al.
Published: (2022)

Development And Evaluation Of Multiplex Real-Time Pcr For Porcine Dna Detection In Processed Food
by: Tan, Lee Lee
Published: (2018)

Detection and quantification of probiotic bacteria using optimized DNA extraction, traditional and real-time PCR methods in complex microbial communities
by: Abdulamir, Ahmed Sahib, et al.
Published: (2010)

Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa)
by: Bilung, Lesley Maurice, et al.
Published: (2005)

Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR.
by: Chai, Fung Pui, et al.
Published: (2011)

Expression analysis using reverse transcription quantitative Real-Time PCR (RT-qPCR) suggests different strategies in Parageobacillus caldoxylosilyticus ER4B under exposure to cold shock
by: Ching, Xin Jie, et al.
Published: (2022)

Expression analysis using reverse transcription quantitative Real-Time PCR (RT-qPCR) suggests different strategies in Parageobacillus caldoxylosilyticus ER4B under exposure to cold shock
by: Jie, Ching Xin, et al.
Published: (2022)

The development of real-time pcr for the detection of bacteraemia
by: Shieng, Gooi Choo
Published: (2016)

Molecular characterisation of Vibrio parahaemolyticus carrying tdh and trh genes using ERIC-, RAPD- and BOX-PCR on local Malaysia bloody clam and Lala
by: Cheah, Yoke Kqueen, et al.
Published: (2013)

Molecular beacon-based real-time PCR method for detection of porcine DNA in gelatin and gelatin capsules
by: Mohamad, N. A., et al.
Published: (2018)

A DNA sequence design for molecular computation of HPP with output visualization based on real-time PCR
by: Ibrahim, Zuwairie, et al.
Published: (2008)

GMO, safety concerns and international trade: developing countries perspective
by: Lakshman, Lekha, et al.
Published: (2011)

Development of DNA Based Technologies for Detection of Genetically Modified Organisms
by: Cheah, Yoke Kqueen
Published: (2006)

Method optimizing DNA extraction on dairy products by real-time PCR targeting porcine-specific mitochondrial DNA gene for halal verification
by: Rosman, Nurul Natasha, et al.
Published: (2014)

Biosensor DNA voltametri berasaskan nanozarah emas bersalut elektrod bercetak skrin karbon untuk pengesanan DNA organisma terubah suai genetik (GMO)
by: Nahdya Khairani,, et al.
Published: (2022)

Molecular detection of Mycoplasma gallisepticum by real time PCR
by: Farhana Yasmin,, et al.
Published: (2014)

Mitochondrial DNA polymorphism in seven cattle breeds in Malaysia
by: Yow, Weng Kit
Published: (2012)

Safety assessment on GMO products: a review / Umi Sarah Khadri
by: Khadri, Umi Sarah
Published: (2018)

Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
by: Zulkifli, Y., et al.
Published: (2009)

A DNA sequence design for molecular computation of hamiltonian path problem with output visualization based on real-time PCR
by: Ibrahim, Zuwairie, et al.
Published: (2008)

Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia
by: Yusop, Zulkifli, et al.
Published: (2009)

Using RAPD-PCR as molecular assessment on the performance of CHROMAgar™ Listeria and PALCAM agar on isolation of Listeria spp. and L. monocytogenes from foods
by: Lee, Hai Yen, et al.
Published: (2011)

Extraction and isolation of PCR amplifiable genomic DNA from Camellia sinensis (L.) Kuntze
by: Umar, Kabir Mustapha, et al.
Published: (2011)

Comparison of PCR fingerprinting techniques for the discrimination of Salmonella enterica subsp. enterica serovar Weltevreden isolated from indigenous vegetables in Malaysia.
by: Cheah, Yoke Kqueen, et al.
Published: (2008)

Molecular quantitation and characterization of Vibrio cholerae from different seafood obtained from wetmarket and supermarket
by: Letchumanan, Vengadesh, et al.
Published: (2012)

MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products.
by: Yew, John Huat Tang, et al.
Published: (2010)

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
by: Paul Cliff, Simon Divis, et al.
Published: (2010)

Multiplex PCR for the concurrent detection and differentiation of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium.
by: Chai, Fung Pui, et al.
Published: (2011)

Detection of Listeria monocytogenes in foods
by: Ponniah, Jeyaletchumi, et al.
Published: (2010)