Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus
The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Humana Press
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16427/ http://psasir.upm.edu.my/id/eprint/16427/1/Rapid%20and%20effective%20method%20of%20RNA%20isolation%20from%20green%20microalga%20Ankistrodesmus%20convolutus.pdf |
| _version_ | 1848842959710060544 |
|---|---|
| author | Thanh, Tran Omar, Hishamuddin Abdullah, Mohd Puad Chi, Vu Thi Quynh Noroozi, Mostafa Ky, Huynh Napis, Suhaimi |
| author_facet | Thanh, Tran Omar, Hishamuddin Abdullah, Mohd Puad Chi, Vu Thi Quynh Noroozi, Mostafa Ky, Huynh Napis, Suhaimi |
| author_sort | Thanh, Tran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The rapid and effective method for the isolation
of RNA from green microalga Ankistrodesmus convolutus
based on homogenization in a simple CTAB buffer and
selective precipitation of RNA with lithium chloride is
developed. This procedure avoids the use of toxic chaotropic
agents and phenol while high concentration of
dithiothreitol is used to inhibit RNase activity and prevent
oxidative cross-linking of nucleic acids by phenolics. The
extraction procedure was able to produce high quality and
intact RNA from A. convolutus. The yield of total RNA
was 0.69–0.73 mg/g of fresh weight, with A260/A280 ratio
of 1.79–1.86. The obtained RNA was of sufficient quality
and suitable for downstream application such as RT-PCR
and cDNA library construction. The procedure may also
have wider applicability for total RNA isolation from other
green microalgae species. |
| first_indexed | 2025-11-15T08:07:25Z |
| format | Article |
| id | upm-16427 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:25Z |
| publishDate | 2009 |
| publisher | Humana Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164272017-09-28T08:03:35Z http://psasir.upm.edu.my/id/eprint/16427/ Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus Thanh, Tran Omar, Hishamuddin Abdullah, Mohd Puad Chi, Vu Thi Quynh Noroozi, Mostafa Ky, Huynh Napis, Suhaimi The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high concentration of dithiothreitol is used to inhibit RNase activity and prevent oxidative cross-linking of nucleic acids by phenolics. The extraction procedure was able to produce high quality and intact RNA from A. convolutus. The yield of total RNA was 0.69–0.73 mg/g of fresh weight, with A260/A280 ratio of 1.79–1.86. The obtained RNA was of sufficient quality and suitable for downstream application such as RT-PCR and cDNA library construction. The procedure may also have wider applicability for total RNA isolation from other green microalgae species. Humana Press 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16427/1/Rapid%20and%20effective%20method%20of%20RNA%20isolation%20from%20green%20microalga%20Ankistrodesmus%20convolutus.pdf Thanh, Tran and Omar, Hishamuddin and Abdullah, Mohd Puad and Chi, Vu Thi Quynh and Noroozi, Mostafa and Ky, Huynh and Napis, Suhaimi (2009) Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus. Molecular Biotechnology, 43 (2). pp. 148-153. ISSN 1073-6085; ESSN: 1559-0305 10.1007/s12033-009-9182-8 |
| spellingShingle | Thanh, Tran Omar, Hishamuddin Abdullah, Mohd Puad Chi, Vu Thi Quynh Noroozi, Mostafa Ky, Huynh Napis, Suhaimi Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title | Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title_full | Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title_fullStr | Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title_full_unstemmed | Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title_short | Rapid and effective method of RNA isolation from green microalga Ankistrodesmus convolutus |
| title_sort | rapid and effective method of rna isolation from green microalga ankistrodesmus convolutus |
| url | http://psasir.upm.edu.my/id/eprint/16427/ http://psasir.upm.edu.my/id/eprint/16427/ http://psasir.upm.edu.my/id/eprint/16427/1/Rapid%20and%20effective%20method%20of%20RNA%20isolation%20from%20green%20microalga%20Ankistrodesmus%20convolutus.pdf |