RAPD analysis of three deer species in Malaysia
The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16425/ http://psasir.upm.edu.my/id/eprint/16425/1/RAPD%20analysis%20of%20three%20deer%20species%20in%20Malaysia.pdf |
| _version_ | 1848842959160606720 |
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| author | El-Jaafari, Habiba A. A. Panandam, Jothi Malar Idris, Ismail Siraj, Siti Shapor |
| author_facet | El-Jaafari, Habiba A. A. Panandam, Jothi Malar Idris, Ismail Siraj, Siti Shapor |
| author_sort | El-Jaafari, Habiba A. A. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively. |
| first_indexed | 2025-11-15T08:07:25Z |
| format | Article |
| id | upm-16425 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:25Z |
| publishDate | 2008 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164252016-12-19T08:20:10Z http://psasir.upm.edu.my/id/eprint/16425/ RAPD analysis of three deer species in Malaysia El-Jaafari, Habiba A. A. Panandam, Jothi Malar Idris, Ismail Siraj, Siti Shapor The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively. Asian-Australasian Association of Animal Production Societies 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16425/1/RAPD%20analysis%20of%20three%20deer%20species%20in%20Malaysia.pdf El-Jaafari, Habiba A. A. and Panandam, Jothi Malar and Idris, Ismail and Siraj, Siti Shapor (2008) RAPD analysis of three deer species in Malaysia. Asian-Australasian Journal of Animal Sciences, 21 (9). pp. 1233-1237. ISSN 1011-2367; ESSN: 1976-5517 http://www.ajas.info/journal/view.php?number=21910 10.5713/ajas.2008.60227 |
| spellingShingle | El-Jaafari, Habiba A. A. Panandam, Jothi Malar Idris, Ismail Siraj, Siti Shapor RAPD analysis of three deer species in Malaysia |
| title | RAPD analysis of three deer species in Malaysia |
| title_full | RAPD analysis of three deer species in Malaysia |
| title_fullStr | RAPD analysis of three deer species in Malaysia |
| title_full_unstemmed | RAPD analysis of three deer species in Malaysia |
| title_short | RAPD analysis of three deer species in Malaysia |
| title_sort | rapd analysis of three deer species in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/16425/ http://psasir.upm.edu.my/id/eprint/16425/ http://psasir.upm.edu.my/id/eprint/16425/ http://psasir.upm.edu.my/id/eprint/16425/1/RAPD%20analysis%20of%20three%20deer%20species%20in%20Malaysia.pdf |