Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR
The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16411/ http://psasir.upm.edu.my/id/eprint/16411/1/16411.pdf |
| _version_ | 1848842955116249088 |
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| author | Ponniah, Jeyaletchumi Robin, Tunung S. P., Margaret Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar |
| author_facet | Ponniah, Jeyaletchumi Robin, Tunung S. P., Margaret Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar |
| author_sort | Ponniah, Jeyaletchumi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and naturally contaminated samples. Based on recovery of L. monocytogenes from artifcially contaminated samples, MPN-PCR showed a moderate correlation (R=0.67) between spiking concentration and microbial levels which was better than the FDA-BAM method (R=0.642) and ISO 11290-2:1998 method (R=0.655). With naturally contaminated samples, it was found that L. monocytogenes was detected in 25% of the vegetable samples using MPN-PCR; 15% of the samples by the FDA-BAM method and 8% of samples using ISO 11290-2:1998 method. Overall, MPN-PCR was found to be a rapid and reliable method that could facilitate the enumeration of L. monocytogenes in vegetables. |
| first_indexed | 2025-11-15T08:07:21Z |
| format | Article |
| id | upm-16411 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:21Z |
| publishDate | 2010 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164112015-09-25T07:54:05Z http://psasir.upm.edu.my/id/eprint/16411/ Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR Ponniah, Jeyaletchumi Robin, Tunung S. P., Margaret Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar The aim of this study was to assess the most probable number-polymerase chain reaction (MPN-PCR) technique for detection of Listeria monocytogenes in salad vegetables in comparison with reference EN ISO 11290-2 and Food Drug Administration Bacteriological Analytical Manual method using artifcial and naturally contaminated samples. Based on recovery of L. monocytogenes from artifcially contaminated samples, MPN-PCR showed a moderate correlation (R=0.67) between spiking concentration and microbial levels which was better than the FDA-BAM method (R=0.642) and ISO 11290-2:1998 method (R=0.655). With naturally contaminated samples, it was found that L. monocytogenes was detected in 25% of the vegetable samples using MPN-PCR; 15% of the samples by the FDA-BAM method and 8% of samples using ISO 11290-2:1998 method. Overall, MPN-PCR was found to be a rapid and reliable method that could facilitate the enumeration of L. monocytogenes in vegetables. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16411/1/16411.pdf Ponniah, Jeyaletchumi and Robin, Tunung and S. P., Margaret and Radu, Son and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Malakar, Pradeep Kumar (2010) Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR. International Food Research Journal, 17 (2). pp. 281-286. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2802%29%202010/IFRJ-2010-281-286_Jeya_Malaysia%5B1%5D.pdf |
| spellingShingle | Ponniah, Jeyaletchumi Robin, Tunung S. P., Margaret Radu, Son Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Malakar, Pradeep Kumar Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title | Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title_full | Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title_fullStr | Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title_full_unstemmed | Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title_short | Quantification of Listeria monocytogenes in salad vegetables by MPN-PCR |
| title_sort | quantification of listeria monocytogenes in salad vegetables by mpn-pcr |
| url | http://psasir.upm.edu.my/id/eprint/16411/ http://psasir.upm.edu.my/id/eprint/16411/ http://psasir.upm.edu.my/id/eprint/16411/1/16411.pdf |