Quantitation of green fluorescent protein using a gel-based imaging method.
Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the qua...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier Masson
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16409/ http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf |
| _version_ | 1848842954561552384 |
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| author | Chew, Few Ne Tan, Wen Siang Ling, Tau Chuan Tan, Chon Seng Tey, Beng Ti |
| author_facet | Chew, Few Ne Tan, Wen Siang Ling, Tau Chuan Tan, Chon Seng Tey, Beng Ti |
| author_sort | Chew, Few Ne |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately.
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| first_indexed | 2025-11-15T08:07:20Z |
| format | Article |
| id | upm-16409 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:07:20Z |
| publishDate | 2009 |
| publisher | Elsevier Masson |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-164092015-11-04T00:58:33Z http://psasir.upm.edu.my/id/eprint/16409/ Quantitation of green fluorescent protein using a gel-based imaging method. Chew, Few Ne Tan, Wen Siang Ling, Tau Chuan Tan, Chon Seng Tey, Beng Ti Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately. Elsevier Masson 2009-01-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf Chew, Few Ne and Tan, Wen Siang and Ling, Tau Chuan and Tan, Chon Seng and Tey, Beng Ti (2009) Quantitation of green fluorescent protein using a gel-based imaging method. Analytical Biochemistry, 384 (2). pp. 353-355. ISSN 0003-2697; ESSN: 1096-0309 English |
| spellingShingle | Chew, Few Ne Tan, Wen Siang Ling, Tau Chuan Tan, Chon Seng Tey, Beng Ti Quantitation of green fluorescent protein using a gel-based imaging method. |
| title | Quantitation of green fluorescent protein using a gel-based imaging method.
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| title_full | Quantitation of green fluorescent protein using a gel-based imaging method.
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| title_fullStr | Quantitation of green fluorescent protein using a gel-based imaging method.
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| title_full_unstemmed | Quantitation of green fluorescent protein using a gel-based imaging method.
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| title_short | Quantitation of green fluorescent protein using a gel-based imaging method.
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| title_sort | quantitation of green fluorescent protein using a gel-based imaging method. |
| url | http://psasir.upm.edu.my/id/eprint/16409/ http://psasir.upm.edu.my/id/eprint/16409/1/Quantitation%20of%20green%20fluorescent%20protein%20using%20a%20gel.pdf |