Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium ph...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16396/ |
| _version_ | 1848842950665043968 |
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| author | Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling |
| author_facet | Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling |
| author_sort | Ooi, Chien Wei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%. |
| first_indexed | 2025-11-15T08:07:17Z |
| format | Article |
| id | upm-16396 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T08:07:17Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-163962015-09-25T02:25:59Z http://psasir.upm.edu.my/id/eprint/16396/ Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling Alcohol/salt-based aqueous two-phase systems (ATPSs) were used to recover lipase derived from Burkholderia pseudomallei (B. pseudomallei). Nine biphasic systems, comprised of an alcohol-based top phase (ethanol, 2-propanol and 1-propanol) and a salt-based bottom phase (ammonium sulfate, potassium phosphate and sodium citrate), were evaluated for their effectiveness in lipase recovery. The stability of lipase in each of the solutions was tested, and phase diagrams were constructed for each system. The optimum partition efficiency for the purification of lipase was obtained in an ATPS of 16% (w/w) 2-propanol and 16% (w/w) phosphate in the presence of 4.5% (w/v) NaCl. The purified lipase had a purification factor of 13.5 and a yield of 99%. Elsevier 2009-10 Article PeerReviewed Ooi, Chien Wei and Tey, Beng Ti and Hii, Siew Ling and Mustapa Kamal, Siti Mazlina and Chi, John Wei Lan and Ariff, Arbakariya and Tau, Chuan Ling (2009) Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems. Process Biochemistry, 44 (10). pp. 1083-1087. ISSN 1359-5113; ESSN: 1873-3298 http://www.sciencedirect.com/science/article/pii/S1359511309001718 10.1016/j.procbio.2009.05.008 |
| spellingShingle | Ooi, Chien Wei Tey, Beng Ti Hii, Siew Ling Mustapa Kamal, Siti Mazlina Chi, John Wei Lan Ariff, Arbakariya Tau, Chuan Ling Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title | Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title_full | Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title_fullStr | Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title_full_unstemmed | Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title_short | Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| title_sort | purification of lipase derived from burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems |
| url | http://psasir.upm.edu.my/id/eprint/16396/ http://psasir.upm.edu.my/id/eprint/16396/ http://psasir.upm.edu.my/id/eprint/16396/ |