Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli
Expression of an isolated gene in a system that directly translates it into a protein is an important step to study the protein encoded by the gene. The isolated gene can be expressed in vivo by a heterologous system. In this study, a bacteria system was used to translate the Tectona grandis Late E...
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| Format: | Article |
| Language: | English |
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Universiti Putra Malaysia Press
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16380/ http://psasir.upm.edu.my/id/eprint/16380/1/16%20Page%20257-261.pdf |
| _version_ | 1848842946416214016 |
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| author | Basherudin, Norlia Muhammad, Norwati Adnan, Norwati Haron, Mohd Rosli Mohd Saleh, Norihan |
| author_facet | Basherudin, Norlia Muhammad, Norwati Adnan, Norwati Haron, Mohd Rosli Mohd Saleh, Norihan |
| author_sort | Basherudin, Norlia |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Expression of an isolated gene in a system that directly translates it into a protein is an important step to study
the protein encoded by the gene. The isolated gene can be expressed in vivo by a heterologous system. In this study, a bacteria system was used to translate the Tectona grandis Late Elongated Hypocotyl (Tg-LHY) gene, which was isolated from flowering tissues of teak (Tectona grandis). The gene was cloned into the pET 14b vector (Novagen) and transformed into BL 21(DE3)/pLysS and Rosetta 2 expression host cells (Novagen). Rosetta 2 host cell has been found to be a good candidate to express the Tg-LHY protein from plant origin, as it recognizes the codon that was found in plant but rarely used in bacteria. The expressed protein was about an expected size, which was 90 kD. Western blot analysis using antibody against His-tag, which was fused to the Tg-LHY protein, proved that the expressed protein was Tg-LHY protein. |
| first_indexed | 2025-11-15T08:07:13Z |
| format | Article |
| id | upm-16380 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:13Z |
| publishDate | 2008 |
| publisher | Universiti Putra Malaysia Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-163802018-08-23T02:36:39Z http://psasir.upm.edu.my/id/eprint/16380/ Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli Basherudin, Norlia Muhammad, Norwati Adnan, Norwati Haron, Mohd Rosli Mohd Saleh, Norihan Expression of an isolated gene in a system that directly translates it into a protein is an important step to study the protein encoded by the gene. The isolated gene can be expressed in vivo by a heterologous system. In this study, a bacteria system was used to translate the Tectona grandis Late Elongated Hypocotyl (Tg-LHY) gene, which was isolated from flowering tissues of teak (Tectona grandis). The gene was cloned into the pET 14b vector (Novagen) and transformed into BL 21(DE3)/pLysS and Rosetta 2 expression host cells (Novagen). Rosetta 2 host cell has been found to be a good candidate to express the Tg-LHY protein from plant origin, as it recognizes the codon that was found in plant but rarely used in bacteria. The expressed protein was about an expected size, which was 90 kD. Western blot analysis using antibody against His-tag, which was fused to the Tg-LHY protein, proved that the expressed protein was Tg-LHY protein. Universiti Putra Malaysia Press 2008 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/16380/1/16%20Page%20257-261.pdf Basherudin, Norlia and Muhammad, Norwati and Adnan, Norwati and Haron, Mohd Rosli and Mohd Saleh, Norihan (2008) Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli. Pertanika Journal of Tropical Agricultural Science, 31 (2). pp. 257-261. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2031%20(2)%20Aug.%202008/16%20Page%20257-261.pdf |
| spellingShingle | Basherudin, Norlia Muhammad, Norwati Adnan, Norwati Haron, Mohd Rosli Mohd Saleh, Norihan Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title | Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title_full | Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title_fullStr | Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title_full_unstemmed | Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title_short | Protein expression of Late Elongated Hypocotyl (LHY) homolog genes of teak in Escherichia coli |
| title_sort | protein expression of late elongated hypocotyl (lhy) homolog genes of teak in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/16380/ http://psasir.upm.edu.my/id/eprint/16380/ http://psasir.upm.edu.my/id/eprint/16380/1/16%20Page%20257-261.pdf |