Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas

Trichoderma sp. KUPM0001 showed good growth during solid substrate fermentation (SSF)of sagopith residue known as hampas, supplemented with 10% (v/w) of mineral salts solution containing 0.5% (w/v) (83.3mM)urea as nitrogen source and an initial moisture content of 80% (v/w). Mycelium suspension of 1...

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Main Authors: Shahrim, Zaizuhana, Sabaratnam, Vikineswary, Abdul Rahman, Nor'aini, Abd. Aziz, Suraini, Hassan, Mohd Ali, Abdul Karim, Mohamed Ismail
Format: Article
Language:English
Published: Academic Journals 2008
Online Access:http://psasir.upm.edu.my/id/eprint/16363/
http://psasir.upm.edu.my/id/eprint/16363/1/Production%20of%20reducing%20sugars%20by%20Trichoderma%20sp.%20KUPM0001%20during%20solid%20substrate%20fermentation%20of%20sago%20starch%20processing%20waste%20hampas.pdf
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author Shahrim, Zaizuhana
Sabaratnam, Vikineswary
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
author_facet Shahrim, Zaizuhana
Sabaratnam, Vikineswary
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
author_sort Shahrim, Zaizuhana
building UPM Institutional Repository
collection Online Access
description Trichoderma sp. KUPM0001 showed good growth during solid substrate fermentation (SSF)of sagopith residue known as hampas, supplemented with 10% (v/w) of mineral salts solution containing 0.5% (w/v) (83.3mM)urea as nitrogen source and an initial moisture content of 80% (v/w). Mycelium suspension of 10% (v/w) density was used as initial inoculum and SSF was carried out at 25±2°C in static condition over a period of 120h. The parameters optimized include the initial moisture content of the substrate, mineral salts solution, urea concentration, inoculum density, incubation temperature and incubation time. Without optimized condition, the maximum reducing sugar obtained was 24mg mL¯¹ compared to 46 mg mL¯¹ substrate during optimized SSF after 96h incubation. The optimum parameters obtained were 80% (v/w) of initial moisture; 10% (v/w) of inoculums size; 1.0% of urea in 20% (w/v) of mineral solution and incubated at 30±2°C. The enzyme activities using optimized condition gave maximum α-amylase, glucoamylase, carboxymethyl cellulase, filter paperase and β-glucosidase of 3.19, 2.22, 1.66, 1.11 and 1.48 U mL¯¹, respectively.
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institution Universiti Putra Malaysia
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language English
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publishDate 2008
publisher Academic Journals
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spelling upm-163632016-09-02T07:27:01Z http://psasir.upm.edu.my/id/eprint/16363/ Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas Shahrim, Zaizuhana Sabaratnam, Vikineswary Abdul Rahman, Nor'aini Abd. Aziz, Suraini Hassan, Mohd Ali Abdul Karim, Mohamed Ismail Trichoderma sp. KUPM0001 showed good growth during solid substrate fermentation (SSF)of sagopith residue known as hampas, supplemented with 10% (v/w) of mineral salts solution containing 0.5% (w/v) (83.3mM)urea as nitrogen source and an initial moisture content of 80% (v/w). Mycelium suspension of 10% (v/w) density was used as initial inoculum and SSF was carried out at 25±2°C in static condition over a period of 120h. The parameters optimized include the initial moisture content of the substrate, mineral salts solution, urea concentration, inoculum density, incubation temperature and incubation time. Without optimized condition, the maximum reducing sugar obtained was 24mg mL¯¹ compared to 46 mg mL¯¹ substrate during optimized SSF after 96h incubation. The optimum parameters obtained were 80% (v/w) of initial moisture; 10% (v/w) of inoculums size; 1.0% of urea in 20% (w/v) of mineral solution and incubated at 30±2°C. The enzyme activities using optimized condition gave maximum α-amylase, glucoamylase, carboxymethyl cellulase, filter paperase and β-glucosidase of 3.19, 2.22, 1.66, 1.11 and 1.48 U mL¯¹, respectively. Academic Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16363/1/Production%20of%20reducing%20sugars%20by%20Trichoderma%20sp.%20KUPM0001%20during%20solid%20substrate%20fermentation%20of%20sago%20starch%20processing%20waste%20hampas.pdf Shahrim, Zaizuhana and Sabaratnam, Vikineswary and Abdul Rahman, Nor'aini and Abd. Aziz, Suraini and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (2008) Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas. Research Journal of Microbiology, 3 (9). pp. 569-579. ISSN 1816-4935 http://www.scialert.net/abstract/?doi=jm.2008.569.579 10.3923/jm.2008.569.579
spellingShingle Shahrim, Zaizuhana
Sabaratnam, Vikineswary
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title_full Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title_fullStr Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title_full_unstemmed Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title_short Production of reducing sugars by Trichoderma sp. KUPM0001 during solid substrate fermentation of sago starch processing waste hampas
title_sort production of reducing sugars by trichoderma sp. kupm0001 during solid substrate fermentation of sago starch processing waste hampas
url http://psasir.upm.edu.my/id/eprint/16363/
http://psasir.upm.edu.my/id/eprint/16363/
http://psasir.upm.edu.my/id/eprint/16363/
http://psasir.upm.edu.my/id/eprint/16363/1/Production%20of%20reducing%20sugars%20by%20Trichoderma%20sp.%20KUPM0001%20during%20solid%20substrate%20fermentation%20of%20sago%20starch%20processing%20waste%20hampas.pdf