Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension
A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bentham Science Publishers
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16357/ http://psasir.upm.edu.my/id/eprint/16357/1/Production%20of%20adenoviral%20vectors%20in%20293%20cells.pdf |
| _version_ | 1848842939753562112 |
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| author | Negrete, Alejandro Tau, Chuan Ling Lyddiatt, Andrew |
| author_facet | Negrete, Alejandro Tau, Chuan Ling Lyddiatt, Andrew |
| author_sort | Negrete, Alejandro |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium. |
| first_indexed | 2025-11-15T08:07:06Z |
| format | Article |
| id | upm-16357 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:07:06Z |
| publishDate | 2008 |
| publisher | Bentham Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-163572015-12-22T04:45:46Z http://psasir.upm.edu.my/id/eprint/16357/ Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension Negrete, Alejandro Tau, Chuan Ling Lyddiatt, Andrew A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium. Bentham Science Publishers 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16357/1/Production%20of%20adenoviral%20vectors%20in%20293%20cells.pdf Negrete, Alejandro and Tau, Chuan Ling and Lyddiatt, Andrew (2008) Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension. The Open Biotechnology Journal, 2. pp. 29-35. ISSN 1874-0707 http://benthamopen.com/contents/pdf/TOBIOTJ/TOBIOTJ-2-29.pdf 10.2174/1874070700802010029 |
| spellingShingle | Negrete, Alejandro Tau, Chuan Ling Lyddiatt, Andrew Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title | Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title_full | Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title_fullStr | Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title_full_unstemmed | Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title_short | Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| title_sort | production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension |
| url | http://psasir.upm.edu.my/id/eprint/16357/ http://psasir.upm.edu.my/id/eprint/16357/ http://psasir.upm.edu.my/id/eprint/16357/ http://psasir.upm.edu.my/id/eprint/16357/1/Production%20of%20adenoviral%20vectors%20in%20293%20cells.pdf |