Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source

The aims of this study are to optimised the process parameters involved for submerged fermentation of mannanase by Aspergillus niger FTC5003 using palm kernel cake (PKC) as carbon source. The parameters investigated include temperature, agitation speed, inoculum size and PKC concentration. The subme...

Full description

Bibliographic Details
Main Authors: Abd. Aziz, Suraini, Ong, Lisa Gaik Ai, Hassan, Mohd Ali, Abdul Karim, Mohamed Ismail
Format: Article
Language:English
Published: Academic Journals 2008
Online Access:http://psasir.upm.edu.my/id/eprint/16352/
http://psasir.upm.edu.my/id/eprint/16352/1/16352.pdf
_version_ 1848842938042286080
author Abd. Aziz, Suraini
Ong, Lisa Gaik Ai
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
author_facet Abd. Aziz, Suraini
Ong, Lisa Gaik Ai
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
author_sort Abd. Aziz, Suraini
building UPM Institutional Repository
collection Online Access
description The aims of this study are to optimised the process parameters involved for submerged fermentation of mannanase by Aspergillus niger FTC5003 using palm kernel cake (PKC) as carbon source. The parameters investigated include temperature, agitation speed, inoculum size and PKC concentration. The submerged fermentation was carried out for 10 days by using palm kernel cake as the sole carbon source with the addition of nitrogen source. Palm kernel cake can be considered as a suitable carbon source for the enzyme production due to it mainly consist of mannan and galactomannan, which is hemicellulose. The mannanase enzyme production using the optimised fermentation condition, which was conducted at agitation speed of 200 rpm, temperature 35 °C, 1x104 spores mL-1 of inoculum size and 2% of palm kernel cake with productivity of 13.00 U/mL/day.
first_indexed 2025-11-15T08:07:05Z
format Article
id upm-16352
institution Universiti Putra Malaysia
institution_category Local University
language English
last_indexed 2025-11-15T08:07:05Z
publishDate 2008
publisher Academic Journals
recordtype eprints
repository_type Digital Repository
spelling upm-163522017-10-27T03:40:51Z http://psasir.upm.edu.my/id/eprint/16352/ Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source Abd. Aziz, Suraini Ong, Lisa Gaik Ai Hassan, Mohd Ali Abdul Karim, Mohamed Ismail The aims of this study are to optimised the process parameters involved for submerged fermentation of mannanase by Aspergillus niger FTC5003 using palm kernel cake (PKC) as carbon source. The parameters investigated include temperature, agitation speed, inoculum size and PKC concentration. The submerged fermentation was carried out for 10 days by using palm kernel cake as the sole carbon source with the addition of nitrogen source. Palm kernel cake can be considered as a suitable carbon source for the enzyme production due to it mainly consist of mannan and galactomannan, which is hemicellulose. The mannanase enzyme production using the optimised fermentation condition, which was conducted at agitation speed of 200 rpm, temperature 35 °C, 1x104 spores mL-1 of inoculum size and 2% of palm kernel cake with productivity of 13.00 U/mL/day. Academic Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16352/1/16352.pdf Abd. Aziz, Suraini and Ong, Lisa Gaik Ai and Hassan, Mohd Ali and Abdul Karim, Mohamed Ismail (2008) Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source. Asian Journal of Biochemistry, 3 (5). pp. 297-307. ISSN 1815-9923; ESSN: 1815-9931 http://scialert.net/abstract/?doi=ajb.2008.297.307 10.3923/ajb.2008.297.307
spellingShingle Abd. Aziz, Suraini
Ong, Lisa Gaik Ai
Hassan, Mohd Ali
Abdul Karim, Mohamed Ismail
Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title_full Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title_fullStr Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title_full_unstemmed Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title_short Process parameters optimisation of mannanase production from Aspergillus niger FTCC 5003 using palm kernel cake as carbon source
title_sort process parameters optimisation of mannanase production from aspergillus niger ftcc 5003 using palm kernel cake as carbon source
url http://psasir.upm.edu.my/id/eprint/16352/
http://psasir.upm.edu.my/id/eprint/16352/
http://psasir.upm.edu.my/id/eprint/16352/
http://psasir.upm.edu.my/id/eprint/16352/1/16352.pdf