Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level
The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet markets and supermarkets in Malaysia. A combination of the most probable number -polymerase chain reaction (MPN- PCR) method was applied to detect the presence of V. parahaemoly...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
The Korean Society for Applied Microbiology
2010
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/16326/ http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf |
| _version_ | 1848842930421235712 |
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| author | Robin, Tunung Paie, Margaret Selina Ponniah, Jeyaletchumi Chai, Lay Ching Tuan Chilek, Tuan Zainazor Mohamad Ghazali, Farinazleen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Robin, Tunung Paie, Margaret Selina Ponniah, Jeyaletchumi Chai, Lay Ching Tuan Chilek, Tuan Zainazor Mohamad Ghazali, Farinazleen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Robin, Tunung |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The purpose of this study was to investigate the biosafety
of Vibrio parahaemolyticus in raw salad vegetables at wet
markets and supermarkets in Malaysia. A combination of the
most probable number -polymerase chain reaction (MPN-
PCR) method was applied to detect the presence of V.
parahaemolyticus and to enumerate their density in the
food samples. The study analyzed 276 samples of common
vegetables eaten raw in Malaysia (Wild cosmos=8; Japanese
parsley=21; Cabbage=30; Lettuce=16; Indian pennywort=17;
Carrot=31; Sweet potato=29; Tomato=38; Cucumber=28;
Four-winged bean=26; Long bean=32). The samples were
purchased from two supermarkets (A and B) and two wet
markets (C and D). The occurrence of V. parahaemolyticus
detected was 20.65%, with a higher frequency of V.
parahaemolyticus in vegetables obtained from wet markets
(Wet market C=27.27%; Wet Market D=32.05%) compared
with supermarkets (Supermarket A=1.64%; Supermarket
B=16.67%). V. parahaemolyticus was most prevalent in Indian
pennywort (41.18%). The density of V. parahaemolyticus
in all the samples ranged from <3 up to >2,400 MPN/g,
mostly <3 MPN/g concentration. Raw vegetables from wet
markets contained higher levels of V. parahaemolyticus
compared with supermarkets. Although V. parahaemolyticus
was present in raw vegetables, its numbers were low. The
results suggest that raw vegetables act as a transmission
route for V. parahaemolyticus. This study will be the first
biosafety assessment of V. parahaemolyticus in raw vegetables in Malaysia. |
| first_indexed | 2025-11-15T08:06:57Z |
| format | Article |
| id | upm-16326 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:06:57Z |
| publishDate | 2010 |
| publisher | The Korean Society for Applied Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-163262015-11-02T06:39:42Z http://psasir.upm.edu.my/id/eprint/16326/ Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level Robin, Tunung Paie, Margaret Selina Ponniah, Jeyaletchumi Chai, Lay Ching Tuan Chilek, Tuan Zainazor Mohamad Ghazali, Farinazleen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son The purpose of this study was to investigate the biosafety of Vibrio parahaemolyticus in raw salad vegetables at wet markets and supermarkets in Malaysia. A combination of the most probable number -polymerase chain reaction (MPN- PCR) method was applied to detect the presence of V. parahaemolyticus and to enumerate their density in the food samples. The study analyzed 276 samples of common vegetables eaten raw in Malaysia (Wild cosmos=8; Japanese parsley=21; Cabbage=30; Lettuce=16; Indian pennywort=17; Carrot=31; Sweet potato=29; Tomato=38; Cucumber=28; Four-winged bean=26; Long bean=32). The samples were purchased from two supermarkets (A and B) and two wet markets (C and D). The occurrence of V. parahaemolyticus detected was 20.65%, with a higher frequency of V. parahaemolyticus in vegetables obtained from wet markets (Wet market C=27.27%; Wet Market D=32.05%) compared with supermarkets (Supermarket A=1.64%; Supermarket B=16.67%). V. parahaemolyticus was most prevalent in Indian pennywort (41.18%). The density of V. parahaemolyticus in all the samples ranged from <3 up to >2,400 MPN/g, mostly <3 MPN/g concentration. Raw vegetables from wet markets contained higher levels of V. parahaemolyticus compared with supermarkets. Although V. parahaemolyticus was present in raw vegetables, its numbers were low. The results suggest that raw vegetables act as a transmission route for V. parahaemolyticus. This study will be the first biosafety assessment of V. parahaemolyticus in raw vegetables in Malaysia. The Korean Society for Applied Microbiology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf Robin, Tunung and Paie, Margaret Selina and Ponniah, Jeyaletchumi and Chai, Lay Ching and Tuan Chilek, Tuan Zainazor and Mohamad Ghazali, Farinazleen and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2010) Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level. Journal of Microbiology and Biotechnology, 20 (2). pp. 391-396. ISSN 1017-7825 Vibrio parahaemolyticus Salad vegetables Polymerase chain reaction 10.4014/jmb.0908.08009 English |
| spellingShingle | Vibrio parahaemolyticus Salad vegetables Polymerase chain reaction Robin, Tunung Paie, Margaret Selina Ponniah, Jeyaletchumi Chai, Lay Ching Tuan Chilek, Tuan Zainazor Mohamad Ghazali, Farinazleen Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title | Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title_full | Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title_fullStr | Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title_full_unstemmed | Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title_short | Prevalence and quantification of Vibrio parahaemolyticus in raw salad vegetables at retail level |
| title_sort | prevalence and quantification of vibrio parahaemolyticus in raw salad vegetables at retail level |
| topic | Vibrio parahaemolyticus Salad vegetables Polymerase chain reaction |
| url | http://psasir.upm.edu.my/id/eprint/16326/ http://psasir.upm.edu.my/id/eprint/16326/ http://psasir.upm.edu.my/id/eprint/16326/1/Prevalence%20and%20quantification%20of%20Vibrio%20parahaemolyticus%20in%20raw%20salad%20vegetables%20at%20retail%20level.pdf |