Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.)
A protocol was developed for in vitro plantlet regeneration of mangosteen (Garcinia Mangostana L.) from shoot tip, stem and seed explants. Shoot tip explants produced the highest mean number of shoots per explant on media supplemented with 1.0 mg/L 6-benzylaminopurin (BAP) and 0.05 mg/L kinetin (KIN...
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| Format: | Article |
| Language: | English |
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Academic Journals
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16232/ http://psasir.upm.edu.my/id/eprint/16232/1/Plant%20regeneration%20as%20affected%20by%20plant%20growth%20regulators.pdf |
| _version_ | 1848842902535405568 |
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| author | Sirchi, Mohammad Hossein Torabi Abdul Kadir, Mihdzar Abdul Aziz, Maheran Abdul Rashid, Azmi Rafat, Arash Javadi, Mohammad Bagher |
| author_facet | Sirchi, Mohammad Hossein Torabi Abdul Kadir, Mihdzar Abdul Aziz, Maheran Abdul Rashid, Azmi Rafat, Arash Javadi, Mohammad Bagher |
| author_sort | Sirchi, Mohammad Hossein Torabi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A protocol was developed for in vitro plantlet regeneration of mangosteen (Garcinia Mangostana L.) from shoot tip, stem and seed explants. Shoot tip explants produced the highest mean number of shoots per explant on media supplemented with 1.0 mg/L 6-benzylaminopurin (BAP) and 0.05 mg/L kinetin (KIN) (73.3). On medium containing 0.1 mg/L BAP and 0.05 mg/L KIN, the highest mean shoot height reached 1.2 cm. The highest percentage of callus formed (90%) was obtained in treatment containing 0.20 mg/L KIN. The effect of BAP in combination with α-naphthalene acetic acid (NAA) was also studied. On half seed explants, the combination of 4 mg/L (w/v) of BAP with 0.2 mg/L (w/v) of (NAA) produced the highest number of shoots per explant (75). In medium containing 2 mg/L (w/v) of BAP and 0.2 mg/L (w/v) of NAA, the mean shoot height reached 1.79 cm also The highest percentage of callus formation (90%) was obtained on treatment containing 0.5 mg/L (w/v) of NAA. For rooting, pretreatment in different indol-3-butyric acid (IBA) and NAA concentrations and comparison of MS salt strengths were evaluated. Identical pattern occurred on medium containing one-quarter strength MS salt and the highest mean number of roots per explant (1.17) was produced in treatment with 2 mg/L IBA. The highest percentage of root formation was obtained on one-quarter strength MS salt medium containing 0.1 mg/L NAA (90.4%). Rooted plantlets were acclimatized on medium composed of soil + sand + organic matters + vermiculite according to the ratio (2: 2: 1: 1). |
| first_indexed | 2025-11-15T08:06:31Z |
| format | Article |
| id | upm-16232 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:06:31Z |
| publishDate | 2008 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-162322016-05-03T10:09:59Z http://psasir.upm.edu.my/id/eprint/16232/ Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) Sirchi, Mohammad Hossein Torabi Abdul Kadir, Mihdzar Abdul Aziz, Maheran Abdul Rashid, Azmi Rafat, Arash Javadi, Mohammad Bagher A protocol was developed for in vitro plantlet regeneration of mangosteen (Garcinia Mangostana L.) from shoot tip, stem and seed explants. Shoot tip explants produced the highest mean number of shoots per explant on media supplemented with 1.0 mg/L 6-benzylaminopurin (BAP) and 0.05 mg/L kinetin (KIN) (73.3). On medium containing 0.1 mg/L BAP and 0.05 mg/L KIN, the highest mean shoot height reached 1.2 cm. The highest percentage of callus formed (90%) was obtained in treatment containing 0.20 mg/L KIN. The effect of BAP in combination with α-naphthalene acetic acid (NAA) was also studied. On half seed explants, the combination of 4 mg/L (w/v) of BAP with 0.2 mg/L (w/v) of (NAA) produced the highest number of shoots per explant (75). In medium containing 2 mg/L (w/v) of BAP and 0.2 mg/L (w/v) of NAA, the mean shoot height reached 1.79 cm also The highest percentage of callus formation (90%) was obtained on treatment containing 0.5 mg/L (w/v) of NAA. For rooting, pretreatment in different indol-3-butyric acid (IBA) and NAA concentrations and comparison of MS salt strengths were evaluated. Identical pattern occurred on medium containing one-quarter strength MS salt and the highest mean number of roots per explant (1.17) was produced in treatment with 2 mg/L IBA. The highest percentage of root formation was obtained on one-quarter strength MS salt medium containing 0.1 mg/L NAA (90.4%). Rooted plantlets were acclimatized on medium composed of soil + sand + organic matters + vermiculite according to the ratio (2: 2: 1: 1). Academic Journals 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16232/1/Plant%20regeneration%20as%20affected%20by%20plant%20growth%20regulators.pdf Sirchi, Mohammad Hossein Torabi and Abdul Kadir, Mihdzar and Abdul Aziz, Maheran and Abdul Rashid, Azmi and Rafat, Arash and Javadi, Mohammad Bagher (2008) Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.). African Journal of Biotechnology, 7 (15). art. no. 7FA09647905. pp. 2693-2701. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/7FA09647905 |
| spellingShingle | Sirchi, Mohammad Hossein Torabi Abdul Kadir, Mihdzar Abdul Aziz, Maheran Abdul Rashid, Azmi Rafat, Arash Javadi, Mohammad Bagher Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title | Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title_full | Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title_fullStr | Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title_full_unstemmed | Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title_short | Plant regeneration as affected by plant growth regulators (PGR) in mangosteen (Garcinia mangostana L.) |
| title_sort | plant regeneration as affected by plant growth regulators (pgr) in mangosteen (garcinia mangostana l.) |
| url | http://psasir.upm.edu.my/id/eprint/16232/ http://psasir.upm.edu.my/id/eprint/16232/ http://psasir.upm.edu.my/id/eprint/16232/1/Plant%20regeneration%20as%20affected%20by%20plant%20growth%20regulators.pdf |