PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines.
The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16221/ |
| _version_ | 1848842899369754624 |
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| author | Shafee, Norazizah Kaluz, Stefan Ru, Ning Stanbridge, Eric John |
| author_facet | Shafee, Norazizah Kaluz, Stefan Ru, Ning Stanbridge, Eric John |
| author_sort | Shafee, Norazizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N-ras allele), correlated with increased PI3K activity. Constitutive activation of the PI3K pathway in MCH603/PI3Kact cells increased HIF-1α but, surprisingly, decreased CAIX levels. The cell-type specific inhibitory effect on CAIX was confirmed at the transcriptional level whereas epigenetic modifications of CA9 were ruled out. In summary, our data do not substantiate the generalization that PI3K upregulation leads to increased HIF activity. |
| first_indexed | 2025-11-15T08:06:28Z |
| format | Article |
| id | upm-16221 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:06:28Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-162212014-02-11T04:41:11Z http://psasir.upm.edu.my/id/eprint/16221/ PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. Shafee, Norazizah Kaluz, Stefan Ru, Ning Stanbridge, Eric John The phosphatidylinositol 3-kinase/Akt (PI3K) pathway regulates hypoxia-inducible factor (HIF) activity. Higher expression of HIF-1α and carbonic anhydrase IX (CAIX), a hypoxia-inducible gene, in HT10806TG fibrosarcoma cells (mutant N-ras allele), compared to derivative MCH603 cells (deleted mutant N-ras allele), correlated with increased PI3K activity. Constitutive activation of the PI3K pathway in MCH603/PI3Kact cells increased HIF-1α but, surprisingly, decreased CAIX levels. The cell-type specific inhibitory effect on CAIX was confirmed at the transcriptional level whereas epigenetic modifications of CA9 were ruled out. In summary, our data do not substantiate the generalization that PI3K upregulation leads to increased HIF activity. Elsevier 2009-09-08 Article PeerReviewed Shafee, Norazizah and Kaluz, Stefan and Ru, Ning and Stanbridge, Eric John (2009) PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. Cancer Letters, 282 (1). pp. 109-115. ISSN 0304-3835; ESSN: 1872-7980 10.1016/j.canlet.2009.03.004 English |
| spellingShingle | Shafee, Norazizah Kaluz, Stefan Ru, Ning Stanbridge, Eric John PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title | PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title_full | PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title_fullStr | PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title_full_unstemmed | PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title_short | PI3K/Akt activity has variable cell-specific effects on expression of HIF target genes, CAIX and VEGF, in human cancer cell lines. |
| title_sort | pi3k/akt activity has variable cell-specific effects on expression of hif target genes, caix and vegf, in human cancer cell lines. |
| url | http://psasir.upm.edu.my/id/eprint/16221/ http://psasir.upm.edu.my/id/eprint/16221/ |