Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak
Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing ba...
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| Format: | Article |
| Language: | English |
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Faculty of Agriculture, University of Brawijaya, Indonesia
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16206/ http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf |
| _version_ | 1848842895066398720 |
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| author | Sabullah, Mohd Khalizan Rahman, M. F. Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Shukor, Mohd Yunus |
| author_facet | Sabullah, Mohd Khalizan Rahman, M. F. Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Shukor, Mohd Yunus |
| author_sort | Sabullah, Mohd Khalizan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. |
| first_indexed | 2025-11-15T08:06:24Z |
| format | Article |
| id | upm-16206 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:06:24Z |
| publishDate | 2016 |
| publisher | Faculty of Agriculture, University of Brawijaya, Indonesia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-162062016-06-08T01:15:22Z http://psasir.upm.edu.my/id/eprint/16206/ Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak Sabullah, Mohd Khalizan Rahman, M. F. Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Shukor, Mohd Yunus Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34oC. Glucose was the best electron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spectrum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants. Faculty of Agriculture, University of Brawijaya, Indonesia 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf Sabullah, Mohd Khalizan and Rahman, M. F. and Ahmad, Siti Aqlima and Sulaiman, Mohd Rosni and Shukor, Mohd Shukri and Shamaan, Nor Aripin and Shukor, Mohd Yunus (2016) Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak. Agrivita, 38 (1). pp. 1-13. ISSN 0126-0537; ESSN: 2477-8516 http://www.agrivita.ub.ac.id/index.php/agrivita/article/view/654 |
| spellingShingle | Sabullah, Mohd Khalizan Rahman, M. F. Ahmad, Siti Aqlima Sulaiman, Mohd Rosni Shukor, Mohd Shukri Shamaan, Nor Aripin Shukor, Mohd Yunus Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title | Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title_full | Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title_fullStr | Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title_full_unstemmed | Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title_short | Isolation and characterization of a molybdenum-reducing and glyphosate-degrading Klebsiella oxytoca strain Saw-5 in soils from Sarawak |
| title_sort | isolation and characterization of a molybdenum-reducing and glyphosate-degrading klebsiella oxytoca strain saw-5 in soils from sarawak |
| url | http://psasir.upm.edu.my/id/eprint/16206/ http://psasir.upm.edu.my/id/eprint/16206/ http://psasir.upm.edu.my/id/eprint/16206/1/Isolation%20and%20characterization%20of%20a%20molybdenum-reducing%20and%20glyphosate-degrading%20Klebsiella%20oxytoca%20strain%20Saw-5%20in%20soils%20from%20Sarawak.pdf |