Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature

The present study aimed to provide an insight of C. jejuni ATCC 33560 phenotype profiles (carbon sources and sensitivity to osmolytes and pH) using Phenotypic MicroArray (PM) system in response to optimal and suboptimal temperature. C. jejuni ATCC 33560 showed utilization carbon sources from amino a...

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Main Authors: Tang, John Yew Huat, Carlson, J., Mohamad Ghazali, Farinazleen, Abdul Aziz, Saleha, Nishibuchi, Mitsuaki, Nakaguchi, Yoshitsugu, Radu, Son
Format: Article
Language:English
Published: Faculty of Food Science and Technology, Universiti Putra Malaysia 2010
Online Access:http://psasir.upm.edu.my/id/eprint/16182/
http://psasir.upm.edu.my/id/eprint/16182/1/16182.pdf
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author Tang, John Yew Huat
Carlson, J.
Mohamad Ghazali, Farinazleen
Abdul Aziz, Saleha
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Radu, Son
author_facet Tang, John Yew Huat
Carlson, J.
Mohamad Ghazali, Farinazleen
Abdul Aziz, Saleha
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Radu, Son
author_sort Tang, John Yew Huat
building UPM Institutional Repository
collection Online Access
description The present study aimed to provide an insight of C. jejuni ATCC 33560 phenotype profiles (carbon sources and sensitivity to osmolytes and pH) using Phenotypic MicroArray (PM) system in response to optimal and suboptimal temperature. C. jejuni ATCC 33560 showed utilization carbon sources from amino acids and carboxylates but not from sugars. C. jejuni ATCC 33560 is sensitive to NaCl at 2% and above but showed survival in a wide range of food preservatives (sodium lactate, sodium phosphate, sodium benzoate, ammonium sulphate and sodium nitrate). When incubated at suboptimal temperature, no phenotype loss was observed in carbon source plates. Phenotype loss of C. jejuni ATCC 33560 was observed in sodium chloride (1%), sodium sulphate (2-3%), sodium formate (1%), sodium lactate (7-12%), sodium phosphate pH7 (100mM and 200mM), ammonium sulphate pH8 (50mM), sodium nitrate (60mM, 80mM and 100mM), sodium nitrite (10mM), and growth in pH5. The phenotypic profile from present study will provide a better insight related to survival of C. jejuni ATCC 33560.
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spelling upm-161822015-09-25T02:48:45Z http://psasir.upm.edu.my/id/eprint/16182/ Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature Tang, John Yew Huat Carlson, J. Mohamad Ghazali, Farinazleen Abdul Aziz, Saleha Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Radu, Son The present study aimed to provide an insight of C. jejuni ATCC 33560 phenotype profiles (carbon sources and sensitivity to osmolytes and pH) using Phenotypic MicroArray (PM) system in response to optimal and suboptimal temperature. C. jejuni ATCC 33560 showed utilization carbon sources from amino acids and carboxylates but not from sugars. C. jejuni ATCC 33560 is sensitive to NaCl at 2% and above but showed survival in a wide range of food preservatives (sodium lactate, sodium phosphate, sodium benzoate, ammonium sulphate and sodium nitrate). When incubated at suboptimal temperature, no phenotype loss was observed in carbon source plates. Phenotype loss of C. jejuni ATCC 33560 was observed in sodium chloride (1%), sodium sulphate (2-3%), sodium formate (1%), sodium lactate (7-12%), sodium phosphate pH7 (100mM and 200mM), ammonium sulphate pH8 (50mM), sodium nitrate (60mM, 80mM and 100mM), sodium nitrite (10mM), and growth in pH5. The phenotypic profile from present study will provide a better insight related to survival of C. jejuni ATCC 33560. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16182/1/16182.pdf Tang, John Yew Huat and Carlson, J. and Mohamad Ghazali, Farinazleen and Abdul Aziz, Saleha and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Radu, Son (2010) Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature. International Food Research Journal, 17 (4). pp. 837-844. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2804%29%202010/%282%29%20IFRJ-2010-109%20Radu%20S.pdf
spellingShingle Tang, John Yew Huat
Carlson, J.
Mohamad Ghazali, Farinazleen
Abdul Aziz, Saleha
Nishibuchi, Mitsuaki
Nakaguchi, Yoshitsugu
Radu, Son
Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title_full Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title_fullStr Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title_full_unstemmed Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title_short Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature
title_sort phenotypic microarray (pm) profiles (carbon sources and sensitivity to osmolytes and ph) of campylobacter jejuni atcc 33560 in response to temperature
url http://psasir.upm.edu.my/id/eprint/16182/
http://psasir.upm.edu.my/id/eprint/16182/
http://psasir.upm.edu.my/id/eprint/16182/1/16182.pdf