Partition of plasmid DNA in polymer-salt aqueous two phase systems
The partition of plasmid DNA (pDNA) in polyethylene glycol (PEG)–phosphate aqueous two-phase systems (ATPS) is presented. A high molecular weight (HMW) and a low molecular weight (LMW) polymer, PEG-1450 and -300, were used in combination with di-potassium hydrogen phosphate. The experimental results...
| Main Authors: | Luechau, Frank, Tau, Chuan Ling, Lyddiatt, Andrew |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2009
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16053/ http://psasir.upm.edu.my/id/eprint/16053/1/Partition%20of%20plasmid%20DNA%20in%20polymer.pdf |
Similar Items
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt.
by: Luechau, Frank, et al.
Published: (2009)
by: Luechau, Frank, et al.
Published: (2009)
Interfacial partition of plasmid DNA in aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2009)
by: Luechau, Frank, et al.
Published: (2009)
Two-step process for initial capture of plasmid DNA and partial removal of RNA using aqueous two-phase systems.
by: Luechau, Frank, et al.
Published: (2010)
by: Luechau, Frank, et al.
Published: (2010)
A descriptive model and methods for up-scaled process routes for interfacial partition of bioparticles in aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2010)
by: Luechau, Frank, et al.
Published: (2010)
Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2009)
by: Luechau, Frank, et al.
Published: (2009)
Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
by: Luechau, Frank, et al.
Published: (2010)
by: Luechau, Frank, et al.
Published: (2010)
Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
by: Yin, Hui Chow, et al.
Published: (2016)
by: Yin, Hui Chow, et al.
Published: (2016)
Interfacial partitioning behaviour of bovine serum albumin in polymer-salt aqueous two-phase system
by: Chow, Yin Hui, et al.
Published: (2013)
by: Chow, Yin Hui, et al.
Published: (2013)
Redefinition of working aqueous two-phase systems: a generic description for prediction of the effective phase chemical composition for process control and biorecovery
by: Selvakumar, Pitchaivelu, et al.
Published: (2010)
by: Selvakumar, Pitchaivelu, et al.
Published: (2010)
Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system
by: Lin, Yu Kiat, et al.
Published: (2016)
by: Lin, Yu Kiat, et al.
Published: (2016)
AQUEOUS TWO-PHASE POLYMER MIXTURE SYSTEM AS PROTEIN XTRACTION TECHNIQUE. MODELLING OF PROTEIN PARTITIONING BEHAVIOR
by: Ahmad, Murni M
Published: (2007)
by: Ahmad, Murni M
Published: (2007)
Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
by: Ooi, Chien Wei, et al.
Published: (2009)
by: Ooi, Chien Wei, et al.
Published: (2009)
Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
by: Ling, Tau Chuan, et al.
Published: (2006)
by: Ling, Tau Chuan, et al.
Published: (2006)
Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system
by: Chow, Yin Hui
Published: (2016)
by: Chow, Yin Hui
Published: (2016)
Integration of mechanical cell disruption and fluidised bed recovery of G3PDH from unclarified disrupted yeast: a comparative study of the performance of unshielded and polymer shielded dye-ligand chromatography systems
by: Ling, Tau Chuan, et al.
Published: (2005)
by: Ling, Tau Chuan, et al.
Published: (2005)
Separation of cyclodextrins (CDs) using aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2012)
by: Ng, Hui Suan, et al.
Published: (2012)
Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems
by: Sewn, Cen Lo, et al.
Published: (2018)
by: Sewn, Cen Lo, et al.
Published: (2018)
Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems
by: Lin, Yu Kiat, et al.
Published: (2013)
by: Lin, Yu Kiat, et al.
Published: (2013)
Extended NRTL model for PEG + salt based aqueous
two-phase system
by: M., Perumalsamy, et al.
Published: (2010)
by: M., Perumalsamy, et al.
Published: (2010)
Separation of single-walled carbon nanotubes using aqueous two-phase system
by: Tang, Malcolm S. Y., et al.
Published: (2014)
by: Tang, Malcolm S. Y., et al.
Published: (2014)
The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract
by: Ling, Tau Chuan, et al.
Published: (2005)
by: Ling, Tau Chuan, et al.
Published: (2005)
Method for preparing plasmid DNA
by: Mohamed, Farahidah, et al.
Published: (2015)
by: Mohamed, Farahidah, et al.
Published: (2015)
The Sykes-Picot agreements – phase two of the partition process
by: Guediri, Kaoutar
Published: (2016)
by: Guediri, Kaoutar
Published: (2016)
Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system
by: M., Perumalsamy, et al.
Published: (2007)
by: M., Perumalsamy, et al.
Published: (2007)
Recovery of lipase derived from Burkholderia cenocepacia ST8 using sustainable aqueous two-phase flotation composed of recycling hydrophilic organic solvent and inorganic salt
by: Anuar, Mohd Shamsul, et al.
Published: (2013)
by: Anuar, Mohd Shamsul, et al.
Published: (2013)
Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
by: Ng, Hui Suan, et al.
Published: (2011)
by: Ng, Hui Suan, et al.
Published: (2011)
Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
by: Chien, Wei Ooi, et al.
Published: (2011)
by: Chien, Wei Ooi, et al.
Published: (2011)
Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2013)
by: Ng, Hui Suan, et al.
Published: (2013)
Extractive fermentation using aqueous two-phase systems for integrated production and purification of extracellular lipase derived from Burkholderia pseudomallei
by: Ooi, Chien Wei, et al.
Published: (2011)
by: Ooi, Chien Wei, et al.
Published: (2011)
Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH
by: Settu, sarananan, et al.
Published: (2007)
by: Settu, sarananan, et al.
Published: (2007)
Removal of endotoxins from plasmid DNA: Analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA
by: Ongkudon, C., et al.
Published: (2012)
by: Ongkudon, C., et al.
Published: (2012)
Modeling of crude oil biodegradation using two phase partitioning bioreactor
by: Ahmadun, Fakhru'l-Razi, et al.
Published: (2014)
by: Ahmadun, Fakhru'l-Razi, et al.
Published: (2014)
Extraction and purification of green polymers using aqueous two-phase extraction (ATPE)
by: Leong, Yoong Kit
Published: (2018)
by: Leong, Yoong Kit
Published: (2018)
Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells
by: Negrete, Alejandro, et al.
Published: (2008)
by: Negrete, Alejandro, et al.
Published: (2008)
Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension
by: Negrete, Alejandro, et al.
Published: (2008)
by: Negrete, Alejandro, et al.
Published: (2008)
Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation
by: Show, Pau Loke, et al.
Published: (2011)
by: Show, Pau Loke, et al.
Published: (2011)
Recovery of Bacillus cereus cyclodextrin glycosyltransferase using ionic liquid-based aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2014)
by: Ng, Hui Suan, et al.
Published: (2014)
Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
by: Ooi, Chien Wei, et al.
Published: (2009)
by: Ooi, Chien Wei, et al.
Published: (2009)
Phase compositions, molar mass, and temperature effect on densities, viscosities, and liquid-liquid equilibrium of polyethylene glycol and salt-based aqueous two-phase systems
by: M., Perumalsamy, et al.
Published: (2009)
by: M., Perumalsamy, et al.
Published: (2009)
Modelling of Liquid-liquid Equilibrium Behaviour of Aqueous Two-Phase Polymer Mixture System for Protein Extraction
by: Ahmad, Murni M
Published: (2007)
by: Ahmad, Murni M
Published: (2007)
Similar Items
-
Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt.
by: Luechau, Frank, et al.
Published: (2009) -
Interfacial partition of plasmid DNA in aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2009) -
Two-step process for initial capture of plasmid DNA and partial removal of RNA using aqueous two-phase systems.
by: Luechau, Frank, et al.
Published: (2010) -
A descriptive model and methods for up-scaled process routes for interfacial partition of bioparticles in aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2010) -
Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2009)