Optimized lipase catalyzed synthesis of adipate ester in a solvent-free system
Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15995/ http://psasir.upm.edu.my/id/eprint/15995/1/Optimized%20lipase%20catalyzed%20synthesis%20of%20adipate%20ester%20in%20a%20solvent.pdf |
| Summary: | Immobilized Candida antarctica lipase-catalyzed
esterification of adipic acid and oleyl alcohol was
investigated in a solvent-free system (SFS). Optimum
conditions for adipate ester synthesis in a stirred-tank
reactor were determined by the response surface methodology
(RSM) approach with respect to important reaction
parameters including time, temperature, agitation speed,
and amount of enzyme. A high conversion yield was
achieved using low enzyme amounts of 2.5% w/w at 60C,
reaction time of 438 min, and agitation speed of 500 rpm.
The good correlation between predicted value (96.0%) and
actual value (95.5%) implies that the model derived from
RSM allows better understanding of the effect of important
reaction parameters on the lipase-catalyzed synthesis of
adipate ester in an organic solvent-free system. Higher
volumetric productivity compared to a solvent-based system
was also offered by SFS. The results demonstrate that
the solvent-free system is efficient for enzymatic synthesis
of adipate ester. |
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