Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM)
The present study embarked on the objective of optimizing protein extraction from freeze dried fish waste (FD-FW), sardine (Sardina pilchardus). Introduction of freeze drying prior to extraction was aimed at reducing the risk of protein loses in fish waste (FW) during frozen storage before the extra...
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| Format: | Article |
| Language: | English |
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2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15983/ http://psasir.upm.edu.my/id/eprint/15983/1/Optimization%20of%20protein%20extraction%20from%20freeze%20dried%20fish%20waste%20using%20response%20surface%20methodology.pdf |
| _version_ | 1848842832603774976 |
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| author | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina M., Siti Nor Fadhilah |
| author_facet | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina M., Siti Nor Fadhilah |
| author_sort | Husin, Nurdiyana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study embarked on the objective of optimizing protein extraction from freeze dried fish waste (FD-FW), sardine (Sardina pilchardus). Introduction of freeze drying prior to extraction was aimed at reducing the risk of protein loses in fish waste (FW) during frozen storage before the extraction process. Response surface methodology (RSM) was used to study the effect of independent variables, namely time (30-60 minutes), pH (7-11), rotation speed (100-300 rpm), and NaOH: substrate ratio (1-3) on protein extraction from FD-FW. From RSM-generated model, the optimum conditions for extraction of protein from FD-FW were identified to be at pH 10.56 in 48.61 minutes reaction time, with rotation speed of 104.77 rpm and NaOH: substrate ratio of 1.54. Predicted protein yield was 85.02 mg/ml while an experimental protein yield was 83.51 mg/ml as revealed by confirmatory studies.
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| first_indexed | 2025-11-15T08:05:24Z |
| format | Article |
| id | upm-15983 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:05:24Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-159832015-09-17T01:09:14Z http://psasir.upm.edu.my/id/eprint/15983/ Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) Husin, Nurdiyana Mustapa Kamal, Siti Mazlina M., Siti Nor Fadhilah The present study embarked on the objective of optimizing protein extraction from freeze dried fish waste (FD-FW), sardine (Sardina pilchardus). Introduction of freeze drying prior to extraction was aimed at reducing the risk of protein loses in fish waste (FW) during frozen storage before the extraction process. Response surface methodology (RSM) was used to study the effect of independent variables, namely time (30-60 minutes), pH (7-11), rotation speed (100-300 rpm), and NaOH: substrate ratio (1-3) on protein extraction from FD-FW. From RSM-generated model, the optimum conditions for extraction of protein from FD-FW were identified to be at pH 10.56 in 48.61 minutes reaction time, with rotation speed of 104.77 rpm and NaOH: substrate ratio of 1.54. Predicted protein yield was 85.02 mg/ml while an experimental protein yield was 83.51 mg/ml as revealed by confirmatory studies. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15983/1/Optimization%20of%20protein%20extraction%20from%20freeze%20dried%20fish%20waste%20using%20response%20surface%20methodology.pdf Husin, Nurdiyana and Mustapa Kamal, Siti Mazlina and M., Siti Nor Fadhilah (2008) Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM). International Journal of Engineering and Technology, 5 (1). pp. 48-56. |
| spellingShingle | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina M., Siti Nor Fadhilah Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title | Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title_full | Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title_fullStr | Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title_full_unstemmed | Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title_short | Optimization of protein extraction from freeze dried fish waste using response surface methodology (RSM) |
| title_sort | optimization of protein extraction from freeze dried fish waste using response surface methodology (rsm) |
| url | http://psasir.upm.edu.my/id/eprint/15983/ http://psasir.upm.edu.my/id/eprint/15983/1/Optimization%20of%20protein%20extraction%20from%20freeze%20dried%20fish%20waste%20using%20response%20surface%20methodology.pdf |