Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method
Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effe...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15975/ http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf |
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| author | Adnani, Atena Basri, Mahiran Abd. Malek, Emilia Salleh, Abu Bakar Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Raja Abdul Rahman, Raja Noor Zaliha |
| author_facet | Adnani, Atena Basri, Mahiran Abd. Malek, Emilia Salleh, Abu Bakar Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Raja Abdul Rahman, Raja Noor Zaliha |
| author_sort | Adnani, Atena |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effects of reaction parameters including reaction time (7–24 h), enzyme amount (0.05–0.3 g), temperature (30–60 °C), amount of molecular sieve (1–4 g), substrate molar ratio (0.3–1) and xylitol concentration (0.005–0.015 g/ml) on the percentage yield of sugar ester were investigated. The optimum conditions derived via Taguchi method were: reaction time 7 h, temperature 60 °C, amount of enzyme 0.12 g, amount of molecular sieve 2.5 g, substrate molar ratio 1 and xylitol concentration 0.015 g/ml. The actual experimental yield was 96.10% under optimum condition, which compared well to the maximum predicted value of 96.27%. |
| first_indexed | 2025-11-15T08:05:22Z |
| format | Article |
| id | upm-15975 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:05:22Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-159752016-09-02T04:31:37Z http://psasir.upm.edu.my/id/eprint/15975/ Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method Adnani, Atena Basri, Mahiran Abd. Malek, Emilia Salleh, Abu Bakar Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Raja Abdul Rahman, Raja Noor Zaliha Enzymatic synthesis of xylitol fatty acid ester was performed in hexane using Novozym 435 (immobilized Candida antarctica lipase on macroporous resin). Taguchi method based on three levels, six variables L27 orthogonal array robust design was implemented to optimize experimental conditions. The effects of reaction parameters including reaction time (7–24 h), enzyme amount (0.05–0.3 g), temperature (30–60 °C), amount of molecular sieve (1–4 g), substrate molar ratio (0.3–1) and xylitol concentration (0.005–0.015 g/ml) on the percentage yield of sugar ester were investigated. The optimum conditions derived via Taguchi method were: reaction time 7 h, temperature 60 °C, amount of enzyme 0.12 g, amount of molecular sieve 2.5 g, substrate molar ratio 1 and xylitol concentration 0.015 g/ml. The actual experimental yield was 96.10% under optimum condition, which compared well to the maximum predicted value of 96.27%. Elsevier 2010-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf Adnani, Atena and Basri, Mahiran and Abd. Malek, Emilia and Salleh, Abu Bakar and Abdul Rahman, Mohd Basyaruddin and Chaibakhsh, Naz and Raja Abdul Rahman, Raja Noor Zaliha (2010) Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method. Industrial Crops and Products, 31 (2). pp. 350-356. ISSN 0926-6690; ESSN: 1872-633X 10.1016/j.indcrop.2009.12.001 |
| spellingShingle | Adnani, Atena Basri, Mahiran Abd. Malek, Emilia Salleh, Abu Bakar Abdul Rahman, Mohd Basyaruddin Chaibakhsh, Naz Raja Abdul Rahman, Raja Noor Zaliha Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title | Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title_full | Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title_fullStr | Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title_full_unstemmed | Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title_short | Optimization of lipase-catalyzed synthesis of xylitol ester by Taguchi robust design method |
| title_sort | optimization of lipase-catalyzed synthesis of xylitol ester by taguchi robust design method |
| url | http://psasir.upm.edu.my/id/eprint/15975/ http://psasir.upm.edu.my/id/eprint/15975/ http://psasir.upm.edu.my/id/eprint/15975/1/Optimization%20of%20lipase.pdf |