Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM)
Amino acid surfactants are high-value surfactants which have excellent emulsifying characteristics and minimal toxicity to the living body. Enzymatic synthesis of palm kernel amino acid surfactant was optimized by response surface methodology (RSM) using palm kernel olein (PKO) and l(+)-lysine catal...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15974/ http://psasir.upm.edu.my/id/eprint/15974/1/Optimization%20of%20lipase.pdf |
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| author | Bidin, Hasmah Basri, Mahiran Mat Radzi, Salina Ariff, Arbakariya Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_facet | Bidin, Hasmah Basri, Mahiran Mat Radzi, Salina Ariff, Arbakariya Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar |
| author_sort | Bidin, Hasmah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Amino acid surfactants are high-value surfactants which have excellent emulsifying characteristics and minimal toxicity to the living body. Enzymatic synthesis of palm kernel amino acid surfactant was optimized by response surface methodology (RSM) using palm kernel olein (PKO) and l(+)-lysine catalyzed by Lipozyme RM IM. The reaction was performed in batch mode stirred tank reactor (STR) with one multi-bladed impeller. A central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were temperature (A): (40.00–70.00 °C), impeller speed (B): (100.00–400.00 rpm), substrates ratio (C): (1.00–4.00 mmol) and enzyme amount (D): (5.00–8.00 g). The optimum condition derived via RSM at fixed reaction time of 24 h was temperature; 47.50 °C, impeller speed; 323.96 rpm, substrates ratio; 3.25 mmol and enzyme amount; 7.25 g. The experimental yield was 89.03% under the optimum condition, which compared well with the maximum predicted value of 93.77%. |
| first_indexed | 2025-11-15T08:05:22Z |
| format | Article |
| id | upm-15974 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:05:22Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-159742016-09-27T08:07:46Z http://psasir.upm.edu.my/id/eprint/15974/ Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) Bidin, Hasmah Basri, Mahiran Mat Radzi, Salina Ariff, Arbakariya Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Amino acid surfactants are high-value surfactants which have excellent emulsifying characteristics and minimal toxicity to the living body. Enzymatic synthesis of palm kernel amino acid surfactant was optimized by response surface methodology (RSM) using palm kernel olein (PKO) and l(+)-lysine catalyzed by Lipozyme RM IM. The reaction was performed in batch mode stirred tank reactor (STR) with one multi-bladed impeller. A central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were temperature (A): (40.00–70.00 °C), impeller speed (B): (100.00–400.00 rpm), substrates ratio (C): (1.00–4.00 mmol) and enzyme amount (D): (5.00–8.00 g). The optimum condition derived via RSM at fixed reaction time of 24 h was temperature; 47.50 °C, impeller speed; 323.96 rpm, substrates ratio; 3.25 mmol and enzyme amount; 7.25 g. The experimental yield was 89.03% under the optimum condition, which compared well with the maximum predicted value of 93.77%. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15974/1/Optimization%20of%20lipase.pdf Bidin, Hasmah and Basri, Mahiran and Mat Radzi, Salina and Ariff, Arbakariya and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2009) Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM). Industrial Crops and Products, 30 (2). pp. 206-211. ISSN 0926-6690; ESSN: 1872-633X 10.1016/j.indcrop.2009.03.006 |
| spellingShingle | Bidin, Hasmah Basri, Mahiran Mat Radzi, Salina Ariff, Arbakariya Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title | Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title_full | Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title_fullStr | Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title_full_unstemmed | Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title_short | Optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (RSM) |
| title_sort | optimization of lipase-catalyzed synthesis of palm amino acid surfactant using response surface methodology (rsm) |
| url | http://psasir.upm.edu.my/id/eprint/15974/ http://psasir.upm.edu.my/id/eprint/15974/ http://psasir.upm.edu.my/id/eprint/15974/1/Optimization%20of%20lipase.pdf |