Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha.
We employed systematic mixture analysis to determine optimal levels of acetate, propionate, and butyrate for cell growth and polyhydroxyalkanoate (PHA) production by Ralstonia eutropha H16. Butyrate was the preferred acid for robust cell growth and high PHA production. The 3-hydroxyvalerate content...
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15973/ http://psasir.upm.edu.my/id/eprint/15973/1/Optimization%20of%20growth%20media%20components%20for%20polyhydroxyalkanoate.pdf |
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| author | Yang, Yung-Hun Brigham, Chrisstopher J. Budde, Charles F. Boccazzi, Paolo Willis, Laura B. Hassan, Mohd Ali Mohd Yusof, Zainal Abidin Rha, ChoKyun Sinskey, Anthony J. |
| author_facet | Yang, Yung-Hun Brigham, Chrisstopher J. Budde, Charles F. Boccazzi, Paolo Willis, Laura B. Hassan, Mohd Ali Mohd Yusof, Zainal Abidin Rha, ChoKyun Sinskey, Anthony J. |
| author_sort | Yang, Yung-Hun |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | We employed systematic mixture analysis to determine optimal levels of acetate, propionate, and butyrate for cell growth and polyhydroxyalkanoate (PHA) production by Ralstonia eutropha H16. Butyrate was the preferred acid for robust cell growth and high PHA production. The 3-hydroxyvalerate content in the resulting PHA depended on the proportion of propionate initially present in the growth medium. The proportion of acetate dramatically affected the final pH of the growth medium. A model was constructed using our data that predicts the effects of these acids, individually and in combination, on cell dry weight (CDW), PHA content (%CDW), PHA production, 3HV in the polymer, and final culture pH. Cell growth and PHA production improved approximately 1.5-fold over initial conditions when the proportion of butyrate was increased. Optimization of the phosphate buffer content in medium containing higher amounts of butyrate improved cell growth and PHA production more than 4-fold. The validated organic acid mixture analysis model can be used to optimize R. eutropha culture conditions, in order to meet targets for PHA production and/or polymer HV content. By modifying the growth medium made from treated industrial waste, such as palm oil mill effluent, more PHA can be produced. |
| first_indexed | 2025-11-15T08:05:21Z |
| format | Article |
| id | upm-15973 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:05:21Z |
| publishDate | 2010 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-159732015-11-03T00:38:29Z http://psasir.upm.edu.my/id/eprint/15973/ Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Yang, Yung-Hun Brigham, Chrisstopher J. Budde, Charles F. Boccazzi, Paolo Willis, Laura B. Hassan, Mohd Ali Mohd Yusof, Zainal Abidin Rha, ChoKyun Sinskey, Anthony J. We employed systematic mixture analysis to determine optimal levels of acetate, propionate, and butyrate for cell growth and polyhydroxyalkanoate (PHA) production by Ralstonia eutropha H16. Butyrate was the preferred acid for robust cell growth and high PHA production. The 3-hydroxyvalerate content in the resulting PHA depended on the proportion of propionate initially present in the growth medium. The proportion of acetate dramatically affected the final pH of the growth medium. A model was constructed using our data that predicts the effects of these acids, individually and in combination, on cell dry weight (CDW), PHA content (%CDW), PHA production, 3HV in the polymer, and final culture pH. Cell growth and PHA production improved approximately 1.5-fold over initial conditions when the proportion of butyrate was increased. Optimization of the phosphate buffer content in medium containing higher amounts of butyrate improved cell growth and PHA production more than 4-fold. The validated organic acid mixture analysis model can be used to optimize R. eutropha culture conditions, in order to meet targets for PHA production and/or polymer HV content. By modifying the growth medium made from treated industrial waste, such as palm oil mill effluent, more PHA can be produced. Springer Verlag 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15973/1/Optimization%20of%20growth%20media%20components%20for%20polyhydroxyalkanoate.pdf Yang, Yung-Hun and Brigham, Chrisstopher J. and Budde, Charles F. and Boccazzi, Paolo and Willis, Laura B. and Hassan, Mohd Ali and Mohd Yusof, Zainal Abidin and Rha, ChoKyun and Sinskey, Anthony J. (2010) Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Applied Microbiology and Biotechnology, 87 (6). pp. 2037-2045. ISSN 0175-7598 Poly-beta-hydroxyalkanoates. Organic acids. Mixture distributions (Probability theory). English |
| spellingShingle | Poly-beta-hydroxyalkanoates. Organic acids. Mixture distributions (Probability theory). Yang, Yung-Hun Brigham, Chrisstopher J. Budde, Charles F. Boccazzi, Paolo Willis, Laura B. Hassan, Mohd Ali Mohd Yusof, Zainal Abidin Rha, ChoKyun Sinskey, Anthony J. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title | Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title_full | Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title_fullStr | Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title_full_unstemmed | Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title_short | Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. |
| title_sort | optimization of growth media components for polyhydroxyalkanoate (pha) production from organic acids by ralstonia eutropha. |
| topic | Poly-beta-hydroxyalkanoates. Organic acids. Mixture distributions (Probability theory). |
| url | http://psasir.upm.edu.my/id/eprint/15973/ http://psasir.upm.edu.my/id/eprint/15973/1/Optimization%20of%20growth%20media%20components%20for%20polyhydroxyalkanoate.pdf |