Optimisation of transient green fluorescent protein (GFP) gene expression in Phalaenopsis Violacea Orchid mediated by Agrobacterium Tumefaciens-mediated transformation system

QR Code

Optimisation of transient green fluorescent protein (GFP) gene expression in Phalaenopsis Violacea Orchid mediated by Agrobacterium Tumefaciens-mediated transformation system

Numerous transformation factors were successfully optimised to develop a reliable and highly efficient Agrobacterium-mediated transformation into the protocorm-like bodies (PLBs) of Phalaenopsis violacea. The optimisation of factors influencing stable transformation efficiency in new species is v...

Full description

Bibliographic Details
Main Authors:	Julkifle, Advina L., Rathinam, Xavier, Sinniah, Uma Rani, Subramaniam, Sreeramanan
Format:	Article
Language:	English English
Published:	INSInet Publication 2010
Subjects:	Orchid culture. Green fluorescent protein. Agricultural biotechnology
Online Access:	http://psasir.upm.edu.my/id/eprint/15959/ http://psasir.upm.edu.my/id/eprint/15959/1/Optimisation%20of%20transient%20green%20fluorescent%20protein.pdf

Similar Items

A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea orchid
by: Gnasekaran, Pavallekoodi, et al.
Published: (2010)

Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter
by: Yong, Wilson Thau Lim, et al.
Published: (2006)

Establishment of a Biolistic-Mediated Transformation System for an Indigenous Fragrant Orchid, Phalaenopsis Violacea
by: Chew, Yee Chern
Published: (2006)

Agrobacterium-Mediated Transformation Of Dendrobium Sonia-28
by: Julkifle, Advina Lizah
Published: (2012)

Transient Expression of gusA and gfp Gene in Agrobacterium-mediated Banana Transformation Using Single Tiny Meristematic Bud
by: Sreeramanan, Subramaniam, et al.
Published: (2006)

Orchid flora of Fraser's Hill, Malaysia
by: Nordin, Farah Alia Nordin
Published: (2012)

Diversity and conservation assessment of orchids in Gunung Ulu Semangko, Selangor, Malaysia
by: Aqlima, Amiri
Published: (2022)

Studies on the Relative Merits of Popular Commercial Orchid Hybrids and Their Clonal Derivatives
by: Ibrahim, Noraini
Published: (1983)

Diversity of orchids in Gunung Jerai, Kedah, Malaysia
by: Auyob, Nur Adilah
Published: (2016)

Optimization of Agrobacterium-Mediated Transformation Parameters for Melastomatacea Spp. Using Green Fluorescent Protein(GFP) as A Reporter
by: Thau, Wilson Lym Yong, et al.
Published: (2006)

Genetic variation, hybridization, in-vitro germination, and morphometric studies of wild orchids (aerides spp.)
by: Chalaparmal, Sivanaswari
Published: (2015)

Identification and Transcript Analysis of Differentially Expressed Genes from Floral Organs of Pigeon Orchid (Dendrobium Crumenatum)
by: Ng, Boon Zean
Published: (2007)

Optimization of a regeneration system for Spathoglottis plicata blume orchid from seeds and analysis of its biochemical properties
by: Mat Yasin, Zaliyatun Akhma
Published: (2014)

DNA barcoding of endangered peninsular Malaysian slipper orchids, the genus Paphiopedilum
by: Rajaram, Micheal Charles
Published: (2018)

Agrobacterium-mediated genetic transformation of Pjalaenopsis using GFP and GUS reporter genes.
by: Mahmood, Maziah, et al.
Published: (2008)

Enhancers of agrobacterium-mediated transformation of Tibouchina semidecandra selected on the basis of GFP expression
by: Yong, Wilson Thau Lym, et al.
Published: (2010)

Diversity and conservation status of selected rare and endemic limestone orchid species in Padawan, Sarawak
by: Khor, Hong Eng
Published: (2012)

Agrobacterium-Mediated Transformation of the Recalcitrant Vanda Kasem’s Delight Orchid with Higher Efficiency
by: Gnasekaran, Pavallekoodi, et al.
Published: (2014)

Morphological development, in vitro storage, and regeneration of protocorm-like bodies of orchid hybrid Dendrobium Shavin White
by: Bustam, Suryanti
Published: (2013)

Transfer of β-1,3-glucanase gene into banana for tolerance to Fusarium wilt disease race 1 using Agrobacterium-mediated transformation system
by: Subramaniam, Sreeramanan, et al.
Published: (2008)

Cryopreservation of Protocorm-Like Bodies of Orchid Hybrid Dendrobium Kasem Boonchoo White by Vitrification
by: Sipen, Philip
Published: (2000)

Diversity of orchids of the summit region in Genting Highlands, Malaysia
by: Ng, Yong Jin
Published: (2011)

Systematic studies of orchid genus Coelogyne in Peninsular Malaysia
by: Yoh, Kok Hon
Published: (2019)

Fluorescence quenching of optical fiber chemical sensor detecting hazardous dinitrobenzene material
by: Yusufu, Abdullahi Magaji
Published: (2017)

In Planta Agrobacterium tumefaciens transformation of MR 219 rice
by: Zainal Abidin, Zaima Azira, et al.
Published: (2011)

Seed transformation of morinda citrifolia via agrobacterium tumefaciens
by: Fazliza, Mohd. Ali
Published: (2008)

Micropropagation of endangered slipper orchid, Paphiopedilum rothschildianum (Rchb. F) stein
by: Ng, Chyuam Yih
Published: (2011)

A protocol for Agrobacterium-mediated transformation of banana with a rice chitinase gene.
by: Sreeramanan, S., et al.
Published: (2009)

Agrobacterium–Mediated Transformation Of Dendrobium Broga Giant Orchid With Chitinase Gene
by: Mohammad, Jasim Uddain
Published: (2016)

Agrobacterium Tumefaciens-Mediated Transformation Of Cabbage (Brassica Oleracea Subsp. Capitata) Cv. Ky Cross With Athsp101 Gene
by: Rafat, Arash
Published: (2008)

Molecular cloning of two virulence genes from Agrobacterium tumefaciens
by: Goh, Hui Ting
Published: (2015)

Diversity, taxonomy and conservation of orchids in Peninsular Malaysia
by: Besi, Edward Entalai
Published: (2022)

Vase life enhancement of Mokara red orchid with Jatropha curcas L., Psidium guajava L and Andrographis paniculata (Burm.f) wall. ex Nees leaf extracts
by: Rahman, Md. Muklesur
Published: (2014)

Extension of vase life of cut orchids using ozonated water and 1-methylycyclopropene
by: Almasi, Parviz
Published: (2013)

Importance of tissue culture in propagation of orchids - a review.
by: Alang, Zaleha Christine
Published: (1975)

Shoot tip regeneration and optimization of Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleraceae var. italica) cv. Green Marvel
by: Ravanfar, Seyed Ali, et al.
Published: (2015)

Comparison of two selective media for the isolation of Agrobacterium tumefaciens from the soil
by: Oh, Pei Min
Published: (2014)

The use of fluorescence correlation spectroscopy to characterize the molecular mobility of fluorescently labelled G protein-coupled receptors
by: Kilpatrick, Laura E., et al.
Published: (2016)

Cryopreservation of Brassia rex Orchid Shoots Using PVS2 Technique
by: Johari, N., et al.
Published: (2009)

Orchid diversity in disturbed forests of Terengganu and Kelantan, Malaysia, as inference for conservation
by: Besi, Edward Entalai
Published: (2018)