Optimisation of cryopreservation technique in Mokara golden nugget orchid using PVS2 vitrification.

Optimisation of cryopreservation technique in Mokara golden nugget orchid using PVS2 vitrification.

The study was conducted to evaluate the effects of various sucrose concentrations on Mokara orchid shoots (0.5-1.0 and 1.0-1.5 cm) after precultured at 24 and 48 h. The 1.0-1.5 cm of Mokara shoots preculture at 0.25 M sucrose for 48 h was the best condition based on TTC assay and subsequently used f...

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Bibliographic Details
Main Authors: Safrinah, R, Xavier, R, Uma Rani, Sinniah, Sreeramanan, S
Format: Article
Language:English
Published: Academic Journals Inc. 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15956/

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