Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma.
Purpose This study was designed to find a reliable Epstein-Barr virus (EBV) immunoglobulin (Ig) G–based diagnostic/screening test for nasopharyngeal carcinoma (NPC) able to demarcate between the NPC-related seropositivity of EBV IgG antibodies and that of other head and neck cancer (HNCA) and contr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15847/ http://psasir.upm.edu.my/id/eprint/15847/1/Novel%20Epstein.pdf |
| _version_ | 1848842793953263616 |
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| author | Abdulamir, Ahmed Sahib Hafidh, Rand Riadh Abu Bakar, Fatimah Abbas, Kassim Ali |
| author_facet | Abdulamir, Ahmed Sahib Hafidh, Rand Riadh Abu Bakar, Fatimah Abbas, Kassim Ali |
| author_sort | Abdulamir, Ahmed Sahib |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Purpose
This study was designed to find a reliable Epstein-Barr virus (EBV) immunoglobulin (Ig) G–based diagnostic/screening test for nasopharyngeal carcinoma (NPC) able to demarcate between the NPC-related seropositivity of EBV IgG antibodies and that of other head and neck cancer (HNCA) and control groups. The NPC-associated immunosuppression affects EBV IgA much more than IgG, leading to inconsistent detection of NPC using EBV IgA antibodies.
Materials and methods
One hundred twenty-two HNCA patients, 42 NPC, 66 laryngeal carcinoma, and 14 hypopharyngeal carcinoma and 3 groups of 100 control subjects were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to find a specific cutoff value for the NPC-related seropositivity of EBV IgG antibodies.
Results
NPC group showed higher serum level of EBV IgG antibodies than control and other HNCA groups (P < .05). However, the traditional cutoff value, mean + 2 SDs of control subjects, failed to demarcate the seropositives of NPC patients from those of healthy population (P > .05). The new cutoff value, mean + 2 SDs of the seropositives group of control subjects who had already been grouped by the traditional cutoff value, proved successful. It succeeded to demarcate between the NPC-related EBV IgG seropositivity and that issued from the persistent, latent, or reactivated EBV infection in the population (P < .05). The sensitivity/specificity of NPC detection by the new cutoff-based ELISA kit, 76.19% and 86%, was close or higher than that of EBV IgA antibodies.
Conclusion
EBV IgG-based ELISA could be used for the diagnosis of NPC using a new cutoff threshold that excludes the population baseline of EBV IgG seropositivity.
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| first_indexed | 2025-11-15T08:04:47Z |
| format | Article |
| id | upm-15847 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:04:47Z |
| publishDate | 2010 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-158472015-11-03T02:19:54Z http://psasir.upm.edu.my/id/eprint/15847/ Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. Abdulamir, Ahmed Sahib Hafidh, Rand Riadh Abu Bakar, Fatimah Abbas, Kassim Ali Purpose This study was designed to find a reliable Epstein-Barr virus (EBV) immunoglobulin (Ig) G–based diagnostic/screening test for nasopharyngeal carcinoma (NPC) able to demarcate between the NPC-related seropositivity of EBV IgG antibodies and that of other head and neck cancer (HNCA) and control groups. The NPC-associated immunosuppression affects EBV IgA much more than IgG, leading to inconsistent detection of NPC using EBV IgA antibodies. Materials and methods One hundred twenty-two HNCA patients, 42 NPC, 66 laryngeal carcinoma, and 14 hypopharyngeal carcinoma and 3 groups of 100 control subjects were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to find a specific cutoff value for the NPC-related seropositivity of EBV IgG antibodies. Results NPC group showed higher serum level of EBV IgG antibodies than control and other HNCA groups (P < .05). However, the traditional cutoff value, mean + 2 SDs of control subjects, failed to demarcate the seropositives of NPC patients from those of healthy population (P > .05). The new cutoff value, mean + 2 SDs of the seropositives group of control subjects who had already been grouped by the traditional cutoff value, proved successful. It succeeded to demarcate between the NPC-related EBV IgG seropositivity and that issued from the persistent, latent, or reactivated EBV infection in the population (P < .05). The sensitivity/specificity of NPC detection by the new cutoff-based ELISA kit, 76.19% and 86%, was close or higher than that of EBV IgA antibodies. Conclusion EBV IgG-based ELISA could be used for the diagnosis of NPC using a new cutoff threshold that excludes the population baseline of EBV IgG seropositivity. Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15847/1/Novel%20Epstein.pdf Abdulamir, Ahmed Sahib and Hafidh, Rand Riadh and Abu Bakar, Fatimah and Abbas, Kassim Ali (2010) Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. American Journal of Otolaryngology, 31 (6). pp. 410-417. ISSN 0196-0709 http://dx.doi.org/10.1016/j.amjoto.2009.06.006 English |
| spellingShingle | Abdulamir, Ahmed Sahib Hafidh, Rand Riadh Abu Bakar, Fatimah Abbas, Kassim Ali Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title | Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title_full | Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title_fullStr | Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title_full_unstemmed | Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title_short | Novel Epstein-Barr virus immunoglobulin G–based approach for the specific detection of nasopharyngeal carcinoma. |
| title_sort | novel epstein-barr virus immunoglobulin g–based approach for the specific detection of nasopharyngeal carcinoma. |
| url | http://psasir.upm.edu.my/id/eprint/15847/ http://psasir.upm.edu.my/id/eprint/15847/ http://psasir.upm.edu.my/id/eprint/15847/1/Novel%20Epstein.pdf |