Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA
Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14 and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin genes. A new combination of plantaricin EF and plantaricin W bacteriocin structural genes was successfully amplified from all...
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Faculty of Food Technology and Biotechnology, University of Zagreb
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15844/ http://psasir.upm.edu.my/id/eprint/15844/1/Novel%20bacteriocinogenic%20Lactobacillus%20plantarum%20strains%20and%20their%20differentiation%20by%20sequence%20analysis%20of%2016S%20rDNA.pdf |
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| author | Moghadam, Morteza Shojaei Foo, Hooi Ling Leow, Thean Chor Abdul Rahim, Raha Loh, Teck Chwen |
| author_facet | Moghadam, Morteza Shojaei Foo, Hooi Ling Leow, Thean Chor Abdul Rahim, Raha Loh, Teck Chwen |
| author_sort | Moghadam, Morteza Shojaei |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14 and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin genes. A new combination of plantaricin EF and plantaricin W bacteriocin structural genes was successfully amplified from all studied strains, suggesting that they were novel bacteriocin- producing L. plantarum strains. A four-base pair variable region was detected in the
short 16S-23S intergenic spacer regions of the studied strains by a comparative analysis with 17 L. plantarum strains deposited in the GenBank, implying they were new genotypes. The studied L. plantarum strains were subsequently differentiated into four groups on the basis of the detected four-base pair variable region of the short 16S-23S intergenic spacer region. Further analysis of the DNA sequence of 23S-5S intergenic spacer region revealed
only one type of 23S-5S intergenic spacer region present in the studied strains, indicating it was highly conserved among the studied L. plantarum strains. Three randomly
amplified polymorphic DNA experiments using three different combinations of arbitrary primers successfully differentiated the studied L. plantarum strains from each other, confirming they were different strains. In conclusion, the studied L. plantarum strains were shown to be novel bacteriocin producers and high level of strain discrimination could be achieved with a combination of randomly amplified polymorphic DNA analysis and the analysis of the variable region of short 16S-23S intergenic spacer region present in L. plantarum strains. |
| first_indexed | 2025-11-15T08:04:46Z |
| format | Article |
| id | upm-15844 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:04:46Z |
| publishDate | 2010 |
| publisher | Faculty of Food Technology and Biotechnology, University of Zagreb |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-158442015-10-21T04:27:11Z http://psasir.upm.edu.my/id/eprint/15844/ Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA Moghadam, Morteza Shojaei Foo, Hooi Ling Leow, Thean Chor Abdul Rahim, Raha Loh, Teck Chwen Six strains of bacteriocinogenic Lactobacillus plantarum (TL1, RG11, RS5, UL4, RG14 and RI11) isolated from Malaysian foods were investigated for their structural bacteriocin genes. A new combination of plantaricin EF and plantaricin W bacteriocin structural genes was successfully amplified from all studied strains, suggesting that they were novel bacteriocin- producing L. plantarum strains. A four-base pair variable region was detected in the short 16S-23S intergenic spacer regions of the studied strains by a comparative analysis with 17 L. plantarum strains deposited in the GenBank, implying they were new genotypes. The studied L. plantarum strains were subsequently differentiated into four groups on the basis of the detected four-base pair variable region of the short 16S-23S intergenic spacer region. Further analysis of the DNA sequence of 23S-5S intergenic spacer region revealed only one type of 23S-5S intergenic spacer region present in the studied strains, indicating it was highly conserved among the studied L. plantarum strains. Three randomly amplified polymorphic DNA experiments using three different combinations of arbitrary primers successfully differentiated the studied L. plantarum strains from each other, confirming they were different strains. In conclusion, the studied L. plantarum strains were shown to be novel bacteriocin producers and high level of strain discrimination could be achieved with a combination of randomly amplified polymorphic DNA analysis and the analysis of the variable region of short 16S-23S intergenic spacer region present in L. plantarum strains. Faculty of Food Technology and Biotechnology, University of Zagreb 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15844/1/Novel%20bacteriocinogenic%20Lactobacillus%20plantarum%20strains%20and%20their%20differentiation%20by%20sequence%20analysis%20of%2016S%20rDNA.pdf Moghadam, Morteza Shojaei and Foo, Hooi Ling and Leow, Thean Chor and Abdul Rahim, Raha and Loh, Teck Chwen (2010) Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA. Food Technology and Biotechnology, 48 (4). pp. 476-483. ISSN 1330-9862 http://public.carnet.hr/ftbrfd/ Lactobacillus plantarum Bacteriocins |
| spellingShingle | Lactobacillus plantarum Bacteriocins Moghadam, Morteza Shojaei Foo, Hooi Ling Leow, Thean Chor Abdul Rahim, Raha Loh, Teck Chwen Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title | Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title_full | Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title_fullStr | Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title_full_unstemmed | Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title_short | Novel bacteriocinogenic Lactobacillus plantarum strains and their differentiation by sequence analysis of 16S rDNA, 16S-23S and 23S-5S intergenic spacer regions and randomly amplified polymorphic DNA |
| title_sort | novel bacteriocinogenic lactobacillus plantarum strains and their differentiation by sequence analysis of 16s rdna, 16s-23s and 23s-5s intergenic spacer regions and randomly amplified polymorphic dna |
| topic | Lactobacillus plantarum Bacteriocins |
| url | http://psasir.upm.edu.my/id/eprint/15844/ http://psasir.upm.edu.my/id/eprint/15844/ http://psasir.upm.edu.my/id/eprint/15844/1/Novel%20bacteriocinogenic%20Lactobacillus%20plantarum%20strains%20and%20their%20differentiation%20by%20sequence%20analysis%20of%2016S%20rDNA.pdf |