Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting
Two molecular typing methods were used in this study to examine the intra/inter-specific genetic relatedness among the A. hydrophila, A. caviae and A. veronii biovar sobria strains. In the analysis by RAPDPCR and ERIC-PCR, the size for RAPD and ERIC fragments ranged from 0.25 to 10.0 kb with an aver...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15714/ http://psasir.upm.edu.my/id/eprint/15714/1/15714.pdf |
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| author | Abdulhadi, Yousr Noaman Napis, Suhaimi Rahmat Ali, Gulam Rusul Mohamed Alitheen, Noorjahan Banu Radu, Son |
| author_facet | Abdulhadi, Yousr Noaman Napis, Suhaimi Rahmat Ali, Gulam Rusul Mohamed Alitheen, Noorjahan Banu Radu, Son |
| author_sort | Abdulhadi, Yousr Noaman |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Two molecular typing methods were used in this study to examine the intra/inter-specific genetic relatedness among the A. hydrophila, A. caviae and A. veronii biovar sobria strains. In the analysis by RAPDPCR and ERIC-PCR, the size for RAPD and ERIC fragments ranged from 0.25 to 10.0 kb with an average number of sixteen and eight bands, respectively. For the inter-species similarity, RAPD could generate 16 major clusters and 36 single isolates at the similarity of 30%. 10 clusters are significant since it grouped the strains according to their own species, however, ERIC-PCR could discriminate the strains into 4 significant clusters and 64 single isolates at the similarity of 50%. Moreover, there was no genetic similarity between food and environmental strains of Aeromonas sp. isolated from different geographical areas as well as from the same geographical area. Eighty five genotypes among the 85 A. hydrophila, A. caviae and A. veronii biovar sobria isolates were generated using RAPD and ERIC-PCR which indicated that the strains were very diverse. |
| first_indexed | 2025-11-15T08:04:11Z |
| format | Article |
| id | upm-15714 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:04:11Z |
| publishDate | 2010 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-157142015-09-25T07:01:28Z http://psasir.upm.edu.my/id/eprint/15714/ Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting Abdulhadi, Yousr Noaman Napis, Suhaimi Rahmat Ali, Gulam Rusul Mohamed Alitheen, Noorjahan Banu Radu, Son Two molecular typing methods were used in this study to examine the intra/inter-specific genetic relatedness among the A. hydrophila, A. caviae and A. veronii biovar sobria strains. In the analysis by RAPDPCR and ERIC-PCR, the size for RAPD and ERIC fragments ranged from 0.25 to 10.0 kb with an average number of sixteen and eight bands, respectively. For the inter-species similarity, RAPD could generate 16 major clusters and 36 single isolates at the similarity of 30%. 10 clusters are significant since it grouped the strains according to their own species, however, ERIC-PCR could discriminate the strains into 4 significant clusters and 64 single isolates at the similarity of 50%. Moreover, there was no genetic similarity between food and environmental strains of Aeromonas sp. isolated from different geographical areas as well as from the same geographical area. Eighty five genotypes among the 85 A. hydrophila, A. caviae and A. veronii biovar sobria isolates were generated using RAPD and ERIC-PCR which indicated that the strains were very diverse. Faculty of Food Science and Technology, Universiti Putra Malaysia 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15714/1/15714.pdf Abdulhadi, Yousr Noaman and Napis, Suhaimi and Rahmat Ali, Gulam Rusul and Mohamed Alitheen, Noorjahan Banu and Radu, Son (2010) Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting. International Food Research Journal, 17 (3). pp. 691-698. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/17%20%2803%29%202010/IFRJ-2010-691-698%20Yousr%20Malaysia.pdf |
| spellingShingle | Abdulhadi, Yousr Noaman Napis, Suhaimi Rahmat Ali, Gulam Rusul Mohamed Alitheen, Noorjahan Banu Radu, Son Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title | Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title_full | Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title_fullStr | Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title_full_unstemmed | Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title_short | Molecular typing of Aeromonas species using RAPD and ERIC-PCR fingerprinting |
| title_sort | molecular typing of aeromonas species using rapd and eric-pcr fingerprinting |
| url | http://psasir.upm.edu.my/id/eprint/15714/ http://psasir.upm.edu.my/id/eprint/15714/ http://psasir.upm.edu.my/id/eprint/15714/1/15714.pdf |