Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria.
Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph prote...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Medwell Publishing
2008
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/15700/ http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf |
| _version_ | 1848842752076283904 |
|---|---|
| author | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| author_facet | Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri |
| author_sort | Abdullah Al-Haj, Nagi Ahmed |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) Tachypleus tridentatus that is believed to be involved in the binds to the lipopolysaccharide of Escherichia coli, Salmonella and Vibrio cholerae. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of Salmonella, V. cholera and E. coil were hybridized to anti-LPS factor gene found in the biolysate of the marine animals. The wzm and wzt genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a PCR-based technique for detection of the food-borne pathogens particularly Sa Salmonella, V. cholera and E. coil were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed. |
| first_indexed | 2025-11-15T08:04:07Z |
| format | Article |
| id | upm-15700 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:04:07Z |
| publishDate | 2008 |
| publisher | Medwell Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-157002015-12-03T09:04:51Z http://psasir.upm.edu.my/id/eprint/15700/ Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) Tachypleus tridentatus that is believed to be involved in the binds to the lipopolysaccharide of Escherichia coli, Salmonella and Vibrio cholerae. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of Salmonella, V. cholera and E. coil were hybridized to anti-LPS factor gene found in the biolysate of the marine animals. The wzm and wzt genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a PCR-based technique for detection of the food-borne pathogens particularly Sa Salmonella, V. cholera and E. coil were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed. Medwell Publishing 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdul Rahim, Raha and Ishak, Zamri (2008) Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. Research Journal of Biological Sciences, 3 (12). pp. 1392-1397. ISSN 1815-8846 Limulus polyphemus Polymerase chain reaction rjbsci.2008.1392.1397 English |
| spellingShingle | Limulus polyphemus Polymerase chain reaction Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdul Rahim, Raha Ishak, Zamri Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title | Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title_full | Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title_fullStr | Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title_full_unstemmed | Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title_short | Molecular characterization of horseshoe crab anti-lipopolysaccharide factor C-peptide for hybridization-based detection method of gram negative bacteria. |
| title_sort | molecular characterization of horseshoe crab anti-lipopolysaccharide factor c-peptide for hybridization-based detection method of gram negative bacteria. |
| topic | Limulus polyphemus Polymerase chain reaction |
| url | http://psasir.upm.edu.my/id/eprint/15700/ http://psasir.upm.edu.my/id/eprint/15700/ http://psasir.upm.edu.my/id/eprint/15700/1/Molecular%20characterization%20of%20horseshoe%20crab%20anti.pdf |