Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus
In this study, we have analysed the sequence of Dg3 clone using bioinformatic tools, determined copy number of this transcript in the genome of B. napus and expression levels at various tissues/organ. The cDNA contained 307-base pair open reading frame encoding 102 amino acid residues, 60-base pair...
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| Format: | Article |
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American Network for Scientific Information
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15699/ http://psasir.upm.edu.my/id/eprint/15699/1/Molecular%20characterization%20of%20Dg3.pdf |
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| author | Namasivayam, Parameswari Hanke, David E. |
| author_facet | Namasivayam, Parameswari Hanke, David E. |
| author_sort | Namasivayam, Parameswari |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In this study, we have analysed the sequence of Dg3 clone using bioinformatic tools, determined copy number of this transcript in the genome of B. napus and expression levels at various tissues/organ. The cDNA contained 307-base pair open reading frame encoding 102 amino acid residues, 60-base pair 5`-untranslated region and 127-base pair 3`-untranslated region. The predicted mature protein has a molecular weight of 9.2 kDa and is acidic, with a predicted isoelectric point (pI) of 6.2. The Dg3 sequence has all the conserved structural characteristics of plant LTPs and showed highest homology to LTPs from other plant species. The transcripts of Dg3 were detected in all tested tissues but highest expression was in siliques and in vitro embryogenic cultures. Possible roles of Dg3 during somatic embryogenesis and normal plant development are discussed. |
| first_indexed | 2025-11-15T08:04:07Z |
| format | Article |
| id | upm-15699 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:04:07Z |
| publishDate | 2008 |
| publisher | American Network for Scientific Information |
| recordtype | eprints |
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| spelling | upm-156992015-12-22T04:42:09Z http://psasir.upm.edu.my/id/eprint/15699/ Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus Namasivayam, Parameswari Hanke, David E. In this study, we have analysed the sequence of Dg3 clone using bioinformatic tools, determined copy number of this transcript in the genome of B. napus and expression levels at various tissues/organ. The cDNA contained 307-base pair open reading frame encoding 102 amino acid residues, 60-base pair 5`-untranslated region and 127-base pair 3`-untranslated region. The predicted mature protein has a molecular weight of 9.2 kDa and is acidic, with a predicted isoelectric point (pI) of 6.2. The Dg3 sequence has all the conserved structural characteristics of plant LTPs and showed highest homology to LTPs from other plant species. The transcripts of Dg3 were detected in all tested tissues but highest expression was in siliques and in vitro embryogenic cultures. Possible roles of Dg3 during somatic embryogenesis and normal plant development are discussed. American Network for Scientific Information 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15699/1/Molecular%20characterization%20of%20Dg3.pdf Namasivayam, Parameswari and Hanke, David E. (2008) Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus. Journal of Biological Sciences, 8 (5). pp. 846-856. ISSN 1727-3048 Plant proteins - Biotechnology Molecular cloning Brassica napus DOI: 10.3923/jbs.2008.846.856 English |
| spellingShingle | Plant proteins - Biotechnology Molecular cloning Brassica napus Namasivayam, Parameswari Hanke, David E. Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title | Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title_full | Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title_fullStr | Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title_full_unstemmed | Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title_short | Molecular characterization of Dg3, a cDNA that encodes a novel lipid transfer protein in Brassica napus |
| title_sort | molecular characterization of dg3, a cdna that encodes a novel lipid transfer protein in brassica napus |
| topic | Plant proteins - Biotechnology Molecular cloning Brassica napus |
| url | http://psasir.upm.edu.my/id/eprint/15699/ http://psasir.upm.edu.my/id/eprint/15699/ http://psasir.upm.edu.my/id/eprint/15699/1/Molecular%20characterization%20of%20Dg3.pdf |