Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture.
A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363)...
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| Format: | Article |
| Language: | English English |
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Universiti Putra Malaysia
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15698/ http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf |
| _version_ | 1848842751437701120 |
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| author | Le, Vinh Thuc Huynh, Ky Siew, Eng Ooi Napis, Suhaimi Ishak, Zamzuri Namasivayam, Parameswari |
| author_facet | Le, Vinh Thuc Huynh, Ky Siew, Eng Ooi Napis, Suhaimi Ishak, Zamzuri Namasivayam, Parameswari |
| author_sort | Le, Vinh Thuc |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363) isolated from cell suspension culture of oil palm. This clone is predicted to encode a single major open reading frame for a polypeptide of 177 amino acids with a predicted molecular mass of 19.6 kDa. The predicted amino acid sequence does not contain any signal peptide and transmembrane region. Based on Kyte-Doolittle hydropathy profile, this protein is predicted to be a soluble protein. The predicted ORF of clone 583 was 59% identical to an amino acid sequence of an unknown protein from Oryza sativa (Acc. BAD25663). Southern analysis showed that this clone might be a member of a multigene family in the oil palm genome. Gene expression study by real time quantitative RT-PCR showed that transcripts of clone 583 might be present in low abundance.
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| first_indexed | 2025-11-15T08:04:07Z |
| format | Article |
| id | upm-15698 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:04:07Z |
| publishDate | 2008 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-156982015-10-05T07:50:36Z http://psasir.upm.edu.my/id/eprint/15698/ Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. Le, Vinh Thuc Huynh, Ky Siew, Eng Ooi Napis, Suhaimi Ishak, Zamzuri Namasivayam, Parameswari A large quantity of ESTs is available from various cDNA libraries of oil palm. The information from oil palm EST databases has been utilized to identify several interesting sequences for molecular characterization. In this study, we report molecular characterization of clone 583 (Acc. No. EU795363) isolated from cell suspension culture of oil palm. This clone is predicted to encode a single major open reading frame for a polypeptide of 177 amino acids with a predicted molecular mass of 19.6 kDa. The predicted amino acid sequence does not contain any signal peptide and transmembrane region. Based on Kyte-Doolittle hydropathy profile, this protein is predicted to be a soluble protein. The predicted ORF of clone 583 was 59% identical to an amino acid sequence of an unknown protein from Oryza sativa (Acc. BAD25663). Southern analysis showed that this clone might be a member of a multigene family in the oil palm genome. Gene expression study by real time quantitative RT-PCR showed that transcripts of clone 583 might be present in low abundance. Universiti Putra Malaysia 2008-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf Le, Vinh Thuc and Huynh, Ky and Siew, Eng Ooi and Napis, Suhaimi and Ishak, Zamzuri and Namasivayam, Parameswari (2008) Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. Pertanika Journal of Science & Technology, 16 (2). pp. 189-199. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/JST.php English |
| spellingShingle | Le, Vinh Thuc Huynh, Ky Siew, Eng Ooi Napis, Suhaimi Ishak, Zamzuri Namasivayam, Parameswari Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title | Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title_full | Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title_fullStr | Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title_full_unstemmed | Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title_short | Molecular characterization of an unknown protein (Acc. No. EU795363) from the ESTs of oil palm (Elaeis guineensis Jacq.) cell suspension culture. |
| title_sort | molecular characterization of an unknown protein (acc. no. eu795363) from the ests of oil palm (elaeis guineensis jacq.) cell suspension culture. |
| url | http://psasir.upm.edu.my/id/eprint/15698/ http://psasir.upm.edu.my/id/eprint/15698/ http://psasir.upm.edu.my/id/eprint/15698/1/Molecular%20characterization%20of%20an%20unknown%20protein.pdf |