Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm.
Primers from within the coding region were used to capture the 5' regulatory sequence of the mesocarpspecific metallothionein-like gene, MT3-A, via PCR-based genome walking. The amplified 1040 bp genomic fragment was cloned and sequenced. The sequence of the genomic clone showed total homology...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
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Malaysian Palm Oil Board
2008
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/15614/ http://psasir.upm.edu.my/id/eprint/15614/1/Mesocarp.pdf |
| _version_ | 1848842726750027776 |
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| author | Abdullah, Siti Nor Akmar Ramli, Zubaidah |
| author_facet | Abdullah, Siti Nor Akmar Ramli, Zubaidah |
| author_sort | Abdullah, Siti Nor Akmar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Primers from within the coding region were used to capture the 5' regulatory sequence of the mesocarpspecific metallothionein-like gene, MT3-A, via PCR-based genome walking. The amplified 1040 bp genomic fragment was cloned and sequenced. The sequence of the genomic clone showed total homology with the MT3-A cDNA sequence within their overlapping regions. Rapid amplification of 5’-cDNA ends (5’-RACE) was used to determine the full length cDNA sequence and the putative transcription site of the gene. The adenine residue at the 5’-end of the RACE product was chosen as the likely transcription start site. The 986 bp promoter region upstream of the adenine contains putative regulatory elements including a TATA box, an ethylene responsive element in reverse orientation and two I-boxes. Functional analysis of the MT3-A promoter was performed using a transient assay system. Transient expression of ß-glucuronidase (GUS) examined using qualitative histochemical GUS assay can be detected in both oil palm mesocarp and leaf tissue slices bombarded with the pBI221 transformation vector which contains the GUS reporter gene under the control of the constitutive cauliflower mosaic virus (CaMV) 35S promoter. However, when the CaMV-35S promoter was replaced with MT3-A promoter in the transformation vector and used for bombardment, transient expression of GUS was detected in the oil palm mesocarp slices only and not in the leaf tissue. This suggests that the MT3-A promoter can be used to target specific gene expression into oil palm mesocarp tissues. |
| first_indexed | 2025-11-15T08:03:43Z |
| format | Article |
| id | upm-15614 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:03:43Z |
| publishDate | 2008 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-156142015-12-03T08:30:12Z http://psasir.upm.edu.my/id/eprint/15614/ Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. Abdullah, Siti Nor Akmar Ramli, Zubaidah Primers from within the coding region were used to capture the 5' regulatory sequence of the mesocarpspecific metallothionein-like gene, MT3-A, via PCR-based genome walking. The amplified 1040 bp genomic fragment was cloned and sequenced. The sequence of the genomic clone showed total homology with the MT3-A cDNA sequence within their overlapping regions. Rapid amplification of 5’-cDNA ends (5’-RACE) was used to determine the full length cDNA sequence and the putative transcription site of the gene. The adenine residue at the 5’-end of the RACE product was chosen as the likely transcription start site. The 986 bp promoter region upstream of the adenine contains putative regulatory elements including a TATA box, an ethylene responsive element in reverse orientation and two I-boxes. Functional analysis of the MT3-A promoter was performed using a transient assay system. Transient expression of ß-glucuronidase (GUS) examined using qualitative histochemical GUS assay can be detected in both oil palm mesocarp and leaf tissue slices bombarded with the pBI221 transformation vector which contains the GUS reporter gene under the control of the constitutive cauliflower mosaic virus (CaMV) 35S promoter. However, when the CaMV-35S promoter was replaced with MT3-A promoter in the transformation vector and used for bombardment, transient expression of GUS was detected in the oil palm mesocarp slices only and not in the leaf tissue. This suggests that the MT3-A promoter can be used to target specific gene expression into oil palm mesocarp tissues. Malaysian Palm Oil Board 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15614/1/Mesocarp.pdf Abdullah, Siti Nor Akmar and Ramli, Zubaidah (2008) Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. Journal of Oil Palm Research, 2 (July). pp. 1-8. ISSN 1511-2780 http://palmoilis.mpob.gov.my Molecular biology English |
| spellingShingle | Molecular biology Abdullah, Siti Nor Akmar Ramli, Zubaidah Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title | Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title_full | Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title_fullStr | Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title_full_unstemmed | Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title_short | Mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| title_sort | mesocarp-specific metallothionien-like gene promoter for genetic engineering of oil palm. |
| topic | Molecular biology |
| url | http://psasir.upm.edu.my/id/eprint/15614/ http://psasir.upm.edu.my/id/eprint/15614/ http://psasir.upm.edu.my/id/eprint/15614/1/Mesocarp.pdf |