Medium optimization for chitinase production from Trichoderma virens using central composite design
Medium development for chitinase production by Trichoderma virens was first carried out using conventional method of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Science+Business Media
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/15599/ http://psasir.upm.edu.my/id/eprint/15599/1/Medium%20optimization%20for%20chitinase%20production%20from%20Trichoderma%20virens%20using%20central%20composite%20design.pdf |
| Summary: | Medium development for chitinase production by Trichoderma virens was first carried out using conventional method
of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface
was generated later from the derived model. An experimental design of four variables including various initial pH
values, chitin, ammonium sulphate, and methanol concentrations were created using Design Expert®
Software, Version 6.0. The design consists of 30 experiments, which include 6 replicates at center points. The optimal value for each variable are 3.0 g/L, chitin; 0.1 g/L, ammonium sulphate; 0.4% (v/v), methanol; and initial pH, 4.0 with predicted chitinase activity of 0.1495 U/mL. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.1471 U/mL, which is almost reaching the predicted value. The optimal medium design showed an improvement of chitinase activity of 80.9% compared to activity obtained from the original Absidia medium composition. |
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