Meat species identification and Halal authentication analysis using mitochondrial DNA.
A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identifi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
2009
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| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/15580/ http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf |
| _version_ | 1848842716993028096 |
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| author | Murugaiah, Chandrika Mohd Noor, Zainon Mastakim, Maimunah Bilung, Lesley Maurice Selamat, Jinap Radu, Son |
| author_facet | Murugaiah, Chandrika Mohd Noor, Zainon Mastakim, Maimunah Bilung, Lesley Maurice Selamat, Jinap Radu, Son |
| author_sort | Murugaiah, Chandrika |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed. |
| first_indexed | 2025-11-15T08:03:34Z |
| format | Article |
| id | upm-15580 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:03:34Z |
| publishDate | 2009 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-155802015-10-22T23:35:11Z http://psasir.upm.edu.my/id/eprint/15580/ Meat species identification and Halal authentication analysis using mitochondrial DNA. Murugaiah, Chandrika Mohd Noor, Zainon Mastakim, Maimunah Bilung, Lesley Maurice Selamat, Jinap Radu, Son A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. AluI, BsaJI, RsaI, MseI, and BstUI enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed. Elsevier 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf Murugaiah, Chandrika and Mohd Noor, Zainon and Mastakim, Maimunah and Bilung, Lesley Maurice and Selamat, Jinap and Radu, Son (2009) Meat species identification and Halal authentication analysis using mitochondrial DNA. Meat Science, 83 (1). pp. 57-61. ISSN 0309-1740 Mitochondrial DNA. Halal food. 10.1016/j.meatsci.2009.03.015 English |
| spellingShingle | Mitochondrial DNA. Halal food. Murugaiah, Chandrika Mohd Noor, Zainon Mastakim, Maimunah Bilung, Lesley Maurice Selamat, Jinap Radu, Son Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title | Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title_full | Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title_fullStr | Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title_full_unstemmed | Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title_short | Meat species identification and Halal authentication analysis using mitochondrial DNA. |
| title_sort | meat species identification and halal authentication analysis using mitochondrial dna. |
| topic | Mitochondrial DNA. Halal food. |
| url | http://psasir.upm.edu.my/id/eprint/15580/ http://psasir.upm.edu.my/id/eprint/15580/ http://psasir.upm.edu.my/id/eprint/15580/1/Meat%20species%20identification%20and%20Halal%20authentication%20analysis%20using%20mitochondrial%20DNA.pdf |