Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage.
Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and MTT (3-(4,5-dimethylthiazol-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier Science Limited
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15498/ http://psasir.upm.edu.my/id/eprint/15498/1/Lycopene.pdf |
| _version_ | 1848842694238928896 |
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| author | Kong , Kin Weng Rajab, Nur Fadilah Prasad, K. Nagendra Ismail, Amin Markom, Masturah Tan , Chin Ping |
| author_facet | Kong , Kin Weng Rajab, Nur Fadilah Prasad, K. Nagendra Ismail, Amin Markom, Masturah Tan , Chin Ping |
| author_sort | Kong , Kin Weng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO2 gave a higher yield than solvent extraction (3.15 vs. 0.68mg/100g dried decanter, corresponding to 42.99 and 33.63mg of lycopene). No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200μg/ml). Solvent extract at 25μg/ml (2.32μM lycopene) and SC-CO2 extract at 200μg/ml (5.09μM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200μg/ml; lycopene=18.65μM) or lycopene standard (10μM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases. |
| first_indexed | 2025-11-15T08:03:12Z |
| format | Article |
| id | upm-15498 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:03:12Z |
| publishDate | 2010 |
| publisher | Elsevier Science Limited |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-154982015-09-22T06:13:32Z http://psasir.upm.edu.my/id/eprint/15498/ Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. Kong , Kin Weng Rajab, Nur Fadilah Prasad, K. Nagendra Ismail, Amin Markom, Masturah Tan , Chin Ping Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO2 gave a higher yield than solvent extraction (3.15 vs. 0.68mg/100g dried decanter, corresponding to 42.99 and 33.63mg of lycopene). No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200μg/ml). Solvent extract at 25μg/ml (2.32μM lycopene) and SC-CO2 extract at 200μg/ml (5.09μM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200μg/ml; lycopene=18.65μM) or lycopene standard (10μM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases. Elsevier Science Limited 2010-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15498/1/Lycopene.pdf Kong , Kin Weng and Rajab, Nur Fadilah and Prasad, K. Nagendra and Ismail, Amin and Markom, Masturah and Tan , Chin Ping (2010) Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. Food Chemistry, 123 (4 ). pp. 1142-1148. ISSN 0308-8146; ESSN: 1873-7072 http://www.elsevier.com/ 10.1016/j.foodchem.2010.05.077 English |
| spellingShingle | Kong , Kin Weng Rajab, Nur Fadilah Prasad, K. Nagendra Ismail, Amin Markom, Masturah Tan , Chin Ping Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title | Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title_full | Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title_fullStr | Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title_full_unstemmed | Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title_short | Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. |
| title_sort | lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and dna damage. |
| url | http://psasir.upm.edu.my/id/eprint/15498/ http://psasir.upm.edu.my/id/eprint/15498/ http://psasir.upm.edu.my/id/eprint/15498/ http://psasir.upm.edu.my/id/eprint/15498/1/Lycopene.pdf |