Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique...
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| Format: | Article |
| Language: | English |
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The Korean Society for Biotechnology and Bioengineering
2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15491/ http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf |
| _version_ | 1848842692331569152 |
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| author | Ling, Tau Chuan Lyddiatt, Andrew |
| author_facet | Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort | Ling, Tau Chuan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg. |
| first_indexed | 2025-11-15T08:03:10Z |
| format | Article |
| id | upm-15491 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:03:10Z |
| publishDate | 2006 |
| publisher | The Korean Society for Biotechnology and Bioengineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-154912019-10-04T08:25:05Z http://psasir.upm.edu.my/id/eprint/15491/ Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent Ling, Tau Chuan Lyddiatt, Andrew Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone, PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency, at 6.5% and a specific activity of 10.2 IU/mg. The Korean Society for Biotechnology and Bioengineering 2006 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf Ling, Tau Chuan and Lyddiatt, Andrew (2006) Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent. Biotechnology and Bioprocess Engineering, 11 (1). pp. 84-87. ISSN 1226-8372; ESSN: 1976-3816 https://link.springer.com/article/10.1007/BF02931874 10.1007/BF02931874 |
| spellingShingle | Ling, Tau Chuan Lyddiatt, Andrew Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title | Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title_full | Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title_fullStr | Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title_full_unstemmed | Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title_short | Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent |
| title_sort | effect of polymer shielding on elution of g3pdh bound to dye-ligand adsorbent |
| url | http://psasir.upm.edu.my/id/eprint/15491/ http://psasir.upm.edu.my/id/eprint/15491/ http://psasir.upm.edu.my/id/eprint/15491/ http://psasir.upm.edu.my/id/eprint/15491/1/Effect%20of%20polymer%20shielding%20on%20elution%20of%20G3PDH%20bound%20to%20dye-ligand%20adsorbent.pdf |