Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters
The lipase from Candida antarctica immobilized on an acrylic resin (Novozym 435) was employed for the catalytic reaction of betulinic acid and phthalic anhydride. The influence of different reaction parameters, such as effect of single and mixed solvents, substrate molar ratio, reaction time, temper...
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15462/ http://psasir.upm.edu.my/id/eprint/15462/1/Lipase.pdf |
| _version_ | 1848842684256485376 |
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| author | Moghaddam, Mansour Ghaffari Ahmad @ Amat, Faujan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin |
| author_facet | Moghaddam, Mansour Ghaffari Ahmad @ Amat, Faujan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin |
| author_sort | Moghaddam, Mansour Ghaffari |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The lipase from Candida antarctica immobilized on an acrylic resin (Novozym 435) was employed for the catalytic reaction of betulinic acid and phthalic anhydride. The influence of different reaction parameters, such as effect of single and mixed solvents, substrate molar ratio, reaction time, temperature, amount of enzyme, effect of inorganic bases and effect of substrate support were investigated and optimized. Optimum conditions to produce 3-O-phthalyl- betulinic acid were observed at reaction time; 24 h, temperature; 55°C, amount of enzyme; 176 mg, substrate molar ratio (betulinic acid: phthalic anhydride, 1:1), inorganic base of K2CO3, amount of celite; 170 mg in 1:1 mixture of chloroform and n-hexane as solvent. At optimum conditions, it gave 61.8% of 3-O-phthalyl- betulinic acid. |
| first_indexed | 2025-11-15T08:03:02Z |
| format | Article |
| id | upm-15462 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:03:02Z |
| publishDate | 2010 |
| publisher | Asian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-154622018-10-09T03:29:45Z http://psasir.upm.edu.my/id/eprint/15462/ Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters Moghaddam, Mansour Ghaffari Ahmad @ Amat, Faujan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin The lipase from Candida antarctica immobilized on an acrylic resin (Novozym 435) was employed for the catalytic reaction of betulinic acid and phthalic anhydride. The influence of different reaction parameters, such as effect of single and mixed solvents, substrate molar ratio, reaction time, temperature, amount of enzyme, effect of inorganic bases and effect of substrate support were investigated and optimized. Optimum conditions to produce 3-O-phthalyl- betulinic acid were observed at reaction time; 24 h, temperature; 55°C, amount of enzyme; 176 mg, substrate molar ratio (betulinic acid: phthalic anhydride, 1:1), inorganic base of K2CO3, amount of celite; 170 mg in 1:1 mixture of chloroform and n-hexane as solvent. At optimum conditions, it gave 61.8% of 3-O-phthalyl- betulinic acid. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15462/1/Lipase.pdf Moghaddam, Mansour Ghaffari and Ahmad @ Amat, Faujan and Basri, Mahiran and Abdul Rahman, Mohd Basyaruddin (2010) Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters. Journal of Applied Sciences, 10 (4). pp. 337-342. ISSN 1812-5654; ESSN: 1812-5662 http://scialert.net/abstract/?doi=jas.2010.337.342 10.3923/jas.2010.337.342 |
| spellingShingle | Moghaddam, Mansour Ghaffari Ahmad @ Amat, Faujan Basri, Mahiran Abdul Rahman, Mohd Basyaruddin Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title | Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title_full | Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title_fullStr | Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title_full_unstemmed | Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title_short | Lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| title_sort | lipase-catalyzed esterification of betulinic acid using phthalic anhydride in organic solvent media: study of reaction parameters |
| url | http://psasir.upm.edu.my/id/eprint/15462/ http://psasir.upm.edu.my/id/eprint/15462/ http://psasir.upm.edu.my/id/eprint/15462/ http://psasir.upm.edu.my/id/eprint/15462/1/Lipase.pdf |