Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics
Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate dat...
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| Format: | Article |
| Language: | English |
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MDPI
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15351/ http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf |
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| author | Azizan, Awanis Ahamad Bustamam, Muhammad Safwan Maulidiani, M. Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah |
| author_facet | Azizan, Awanis Ahamad Bustamam, Muhammad Safwan Maulidiani, M. Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah |
| author_sort | Azizan, Awanis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate data analysis (MVDA). The unsupervised data analysis, using principal component analysis (PCA), resolved the five types of extracts made by solvents ranging from polar to non-polar into five different clusters. Collectively, with various extraction solvents, 11 amino acids, cholesterol, 6 fatty acids, 2 sugars, 1 osmolyte, 6 carotenoids and 2 chlorophyll pigments were identified. The fatty acids and both carotenoid pigments as well as chlorophyll, were observed in the extracts made from medium polar (acetone, chloroform) and non-polar (hexane) solvents. It is suggested that the compounds were the characteristic markers that influenced the separation between the clusters. Based on partial least square (PLS) analysis, fucoxanthin, astaxanthin, violaxanthin, zeaxanthin, canthaxanthin, and lutein displayed strong correlation to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) inhibitory activity. This metabolomics study showed that solvent extractions are one of the main bottlenecks for the maximum recovery of bioactive microalgal compounds and could be a better source of natural antioxidants due to a high value of metabolites. |
| first_indexed | 2025-11-15T08:02:33Z |
| format | Article |
| id | upm-15351 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:02:33Z |
| publishDate | 2018 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-153512019-07-08T07:30:21Z http://psasir.upm.edu.my/id/eprint/15351/ Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics Azizan, Awanis Ahamad Bustamam, Muhammad Safwan Maulidiani, M. Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah Microalgae are promising candidate resources from marine ecology for health-improving effects. Metabolite profiling of the microalgal diatom, Chaetoceros calcitrans was conducted by using robust metabolomics tools, namely 1H nuclear magnetic resonance (NMR) spectroscopy coupled with multivariate data analysis (MVDA). The unsupervised data analysis, using principal component analysis (PCA), resolved the five types of extracts made by solvents ranging from polar to non-polar into five different clusters. Collectively, with various extraction solvents, 11 amino acids, cholesterol, 6 fatty acids, 2 sugars, 1 osmolyte, 6 carotenoids and 2 chlorophyll pigments were identified. The fatty acids and both carotenoid pigments as well as chlorophyll, were observed in the extracts made from medium polar (acetone, chloroform) and non-polar (hexane) solvents. It is suggested that the compounds were the characteristic markers that influenced the separation between the clusters. Based on partial least square (PLS) analysis, fucoxanthin, astaxanthin, violaxanthin, zeaxanthin, canthaxanthin, and lutein displayed strong correlation to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) inhibitory activity. This metabolomics study showed that solvent extractions are one of the main bottlenecks for the maximum recovery of bioactive microalgal compounds and could be a better source of natural antioxidants due to a high value of metabolites. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf Azizan, Awanis and Ahamad Bustamam, Muhammad Safwan and Maulidiani, M. and Shaari, Khozirah and Ismail, Intan Safinar and Nagao, Norio and Abas, Faridah (2018) Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics. Marine Drugs, 16 (5). art. no. 154. pp. 1-19. ISSN 1660-3397 https://www.mdpi.com/1660-3397/16/5/154 10.3390/md16050154 |
| spellingShingle | Azizan, Awanis Ahamad Bustamam, Muhammad Safwan Maulidiani, M. Shaari, Khozirah Ismail, Intan Safinar Nagao, Norio Abas, Faridah Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title | Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title_full | Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title_fullStr | Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title_full_unstemmed | Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title_short | Metabolite profiling of the microalgal diatom Chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1H NMR-based metabolomics |
| title_sort | metabolite profiling of the microalgal diatom chaetoceros calcitrans and correlation with antioxidant and nitric oxide inhibitory activities via 1h nmr-based metabolomics |
| url | http://psasir.upm.edu.my/id/eprint/15351/ http://psasir.upm.edu.my/id/eprint/15351/ http://psasir.upm.edu.my/id/eprint/15351/ http://psasir.upm.edu.my/id/eprint/15351/1/15351.pdf |