Isolation of trinucleotide microsatellite markers for Mystus nemurus
Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed t...
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| Format: | Article |
| Language: | English English |
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Springer
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15335/ http://psasir.upm.edu.my/id/eprint/15335/1/Isolation%20of%20trinucleotide%20microsatellite%20markers%20for%20Mystus%20nemurus.pdf |
| _version_ | 1848842648368971776 |
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| author | Hoh , Boon Peng Siraj, Siti Shapor Tan, Soon Guan Yusoff, Khatijah |
| author_facet | Hoh , Boon Peng Siraj, Siti Shapor Tan, Soon Guan Yusoff, Khatijah |
| author_sort | Hoh , Boon Peng |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291). |
| first_indexed | 2025-11-15T08:02:28Z |
| format | Article |
| id | upm-15335 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:02:28Z |
| publishDate | 2008 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-153352015-09-17T01:02:52Z http://psasir.upm.edu.my/id/eprint/15335/ Isolation of trinucleotide microsatellite markers for Mystus nemurus Hoh , Boon Peng Siraj, Siti Shapor Tan, Soon Guan Yusoff, Khatijah Twelve single-locus trinucleotide microsatellite markers were developed to characterize the Asian river catfish, Mystus nemurus, an important food fish in Southeast Asia. They were obtained by using a rapid method, namely, the 5′ anchored PCR enrichment protocol. The specific primers were designed to flank the repeat sequences and these were subsequently used to characterize 90 unrelated fish from Malaysia. The number of alleles per locus ranged from 2 (MnVj2-281) to 12 (MnBp8-4-43b) while the levels of heterozygosity ranged from 0.0444 (MnVj2-1-19) to 0.7458 (MnVj2-291). Springer 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15335/1/Isolation%20of%20trinucleotide%20microsatellite%20markers%20for%20Mystus%20nemurus.pdf Hoh , Boon Peng and Siraj, Siti Shapor and Tan, Soon Guan and Yusoff, Khatijah (2008) Isolation of trinucleotide microsatellite markers for Mystus nemurus. Russian Journal of Genetics, 44 (3). pp. 369-371. ISSN 1022-7954 Catfishes - Research Microsatellites (Genetics) Genetic markers English |
| spellingShingle | Catfishes - Research Microsatellites (Genetics) Genetic markers Hoh , Boon Peng Siraj, Siti Shapor Tan, Soon Guan Yusoff, Khatijah Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title | Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title_full | Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title_fullStr | Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title_full_unstemmed | Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title_short | Isolation of trinucleotide microsatellite markers for Mystus nemurus |
| title_sort | isolation of trinucleotide microsatellite markers for mystus nemurus |
| topic | Catfishes - Research Microsatellites (Genetics) Genetic markers |
| url | http://psasir.upm.edu.my/id/eprint/15335/ http://psasir.upm.edu.my/id/eprint/15335/1/Isolation%20of%20trinucleotide%20microsatellite%20markers%20for%20Mystus%20nemurus.pdf |