Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.

In order to construct cellulolytic fungal mixed cultures, screening and isolation of cellulolytic fungi was done using rotten oil palm fruit bunches as microorganism source. Three isolated fungi had shown the ability to degrade cellulose based on decolorization of CMC selective agar using Gram’s iod...

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Main Authors: Abu Bakar, Nurul Kartini, Abd. Aziz, Suraini, Hassan, Mohd Ali, Mohamad Ghazali, Farinazleen
Format: Article
Language:English
English
Published: Asian Network for Scientific Information (ANSINET) 2010
Subjects:
Online Access:http://psasir.upm.edu.my/id/eprint/15332/
http://psasir.upm.edu.my/id/eprint/15332/1/Isolation%20and%20selection%20of%20appropriate%20cellulolytic%20mixed%20microbial%20cultures%20for%20cellulases%20production%20from%20oil%20palm%20empty%20fruit%20bunch.pdf
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author Abu Bakar, Nurul Kartini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Mohamad Ghazali, Farinazleen
author_facet Abu Bakar, Nurul Kartini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Mohamad Ghazali, Farinazleen
author_sort Abu Bakar, Nurul Kartini
building UPM Institutional Repository
collection Online Access
description In order to construct cellulolytic fungal mixed cultures, screening and isolation of cellulolytic fungi was done using rotten oil palm fruit bunches as microorganism source. Three isolated fungi had shown the ability to degrade cellulose based on decolorization of CMC selective agar using Gram’s iodine as color indicator. However, only two strains; KS1 and KS5 were selected for construction fungal mixed culture. Based on fungal interaction evaluation test done on PDA agar, both strains showed contact deadlock inhibition interaction with each other. In correlation to cellulase enzymes production, mixed cultures of strains KS1 and KS5 showed low enzymes activity compared to pure culture system. Although, the cellulase enzymes production is low, total cellulase enzymes composition was better than in pure culture system individually.
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institution Universiti Putra Malaysia
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language English
English
last_indexed 2025-11-15T08:02:27Z
publishDate 2010
publisher Asian Network for Scientific Information (ANSINET)
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spelling upm-153322015-11-02T04:28:47Z http://psasir.upm.edu.my/id/eprint/15332/ Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch. Abu Bakar, Nurul Kartini Abd. Aziz, Suraini Hassan, Mohd Ali Mohamad Ghazali, Farinazleen In order to construct cellulolytic fungal mixed cultures, screening and isolation of cellulolytic fungi was done using rotten oil palm fruit bunches as microorganism source. Three isolated fungi had shown the ability to degrade cellulose based on decolorization of CMC selective agar using Gram’s iodine as color indicator. However, only two strains; KS1 and KS5 were selected for construction fungal mixed culture. Based on fungal interaction evaluation test done on PDA agar, both strains showed contact deadlock inhibition interaction with each other. In correlation to cellulase enzymes production, mixed cultures of strains KS1 and KS5 showed low enzymes activity compared to pure culture system. Although, the cellulase enzymes production is low, total cellulase enzymes composition was better than in pure culture system individually. Asian Network for Scientific Information (ANSINET) 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15332/1/Isolation%20and%20selection%20of%20appropriate%20cellulolytic%20mixed%20microbial%20cultures%20for%20cellulases%20production%20from%20oil%20palm%20empty%20fruit%20bunch.pdf Abu Bakar, Nurul Kartini and Abd. Aziz, Suraini and Hassan, Mohd Ali and Mohamad Ghazali, Farinazleen (2010) Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch. Biotechnology, 9 (1). pp. 73-78. ISSN 1682-296X Microorganisms 10.3923/biotech.2010.73.78 English
spellingShingle Microorganisms
Abu Bakar, Nurul Kartini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Mohamad Ghazali, Farinazleen
Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title_full Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title_fullStr Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title_full_unstemmed Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title_short Isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
title_sort isolation and selection of appropriate cellulolytic mixed microbial cultures for cellulases production from oil palm empty fruit bunch.
topic Microorganisms
url http://psasir.upm.edu.my/id/eprint/15332/
http://psasir.upm.edu.my/id/eprint/15332/
http://psasir.upm.edu.my/id/eprint/15332/1/Isolation%20and%20selection%20of%20appropriate%20cellulolytic%20mixed%20microbial%20cultures%20for%20cellulases%20production%20from%20oil%20palm%20empty%20fruit%20bunch.pdf