Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica

A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate,...

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Main Authors: Abd. Shukor, Mohd. Yunus, Ali Hassan, Nor Ayshah Alia, Jusoh, Arif Zaidi, Nagar, Perumal, Shamaan, Nor Aripin, Mac Cormack, Walter Patricio, Syed, Mohd Arif
Format: Article
Language:English
Published: Triveni Enterprises 2009
Online Access:http://psasir.upm.edu.my/id/eprint/15320/
http://psasir.upm.edu.my/id/eprint/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf
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author Abd. Shukor, Mohd. Yunus
Ali Hassan, Nor Ayshah Alia
Jusoh, Arif Zaidi
Nagar, Perumal
Shamaan, Nor Aripin
Mac Cormack, Walter Patricio
Syed, Mohd Arif
author_facet Abd. Shukor, Mohd. Yunus
Ali Hassan, Nor Ayshah Alia
Jusoh, Arif Zaidi
Nagar, Perumal
Shamaan, Nor Aripin
Mac Cormack, Walter Patricio
Syed, Mohd Arif
author_sort Abd. Shukor, Mohd. Yunus
building UPM Institutional Repository
collection Online Access
description A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium.
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institution Universiti Putra Malaysia
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language English
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publishDate 2009
publisher Triveni Enterprises
recordtype eprints
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spelling upm-153202018-03-27T08:56:31Z http://psasir.upm.edu.my/id/eprint/15320/ Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica Abd. Shukor, Mohd. Yunus Ali Hassan, Nor Ayshah Alia Jusoh, Arif Zaidi Nagar, Perumal Shamaan, Nor Aripin Mac Cormack, Walter Patricio Syed, Mohd Arif A diesel-degrading bacterium from Antarctica has been isolated. The isolate was tentatively identified as Pseudomonas sp. strain DRYJ3 based on partial 16SrDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Growth on diesel was supported optimally by ammonium sulphate, nitrate and nitrite. The bacterium grew optimally in between 10 and 15°C, pH 7.0 and 3.5% (v/v) diesel. The biodegradation of diesel oil by the strain increased in efficiency from the second to the sixth day of incubation from 1.4 to 18.8% before levelling off on the eighth day n-alkane oxidizing and aldehyde reductase activities were detected in the crude enzyme preparation suggesting the existence of terminal n-alkane oxidizing activity in this bacterium. Triveni Enterprises 2009 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf Abd. Shukor, Mohd. Yunus and Ali Hassan, Nor Ayshah Alia and Jusoh, Arif Zaidi and Nagar, Perumal and Shamaan, Nor Aripin and Mac Cormack, Walter Patricio and Syed, Mohd Arif (2009) Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. Journal of Environmental Biology, 30 (1). pp. 1-6. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=issue_toc&issue=200901_jan09_spl
spellingShingle Abd. Shukor, Mohd. Yunus
Ali Hassan, Nor Ayshah Alia
Jusoh, Arif Zaidi
Nagar, Perumal
Shamaan, Nor Aripin
Mac Cormack, Walter Patricio
Syed, Mohd Arif
Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title_full Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title_fullStr Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title_full_unstemmed Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title_short Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica
title_sort isolation and characterization of a pseudomonas diesel-degrading strain from antarctica
url http://psasir.upm.edu.my/id/eprint/15320/
http://psasir.upm.edu.my/id/eprint/15320/
http://psasir.upm.edu.my/id/eprint/15320/1/Isolation%20and%20characterization%20of%20a%20Pseudomonas%20diesel.pdf