Interfacial partition of plasmid DNA in aqueous two-phase systems

QR Code

Interfacial partition of plasmid DNA in aqueous two-phase systems

In this paper, the partition of plasmid DNA (pDNA) in polyethylene glycol (PEG) 300 and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) under gravity separation was investigated. The influence of temperature (20 and 30 °C) and mode of phase separation (40–60% (w/w) lysate concentrat...

Full description

Bibliographic Details
Main Authors:	Luechau, Frank, Ling, Tau Chuan, Lyddiatt, Andrew
Format:	Article
Language:	English
Published:	Elsevier 2009
Online Access:	http://psasir.upm.edu.my/id/eprint/15267/ http://psasir.upm.edu.my/id/eprint/15267/1/Interfacial%20partition%20of%20plasmid%20DNA%20in%20aqueous%20two-phase%20systems.pdf

Similar Items

Partition of plasmid DNA in polymer-salt aqueous two phase systems
by: Luechau, Frank, et al.
Published: (2009)

Selective partition of plasmid DNA and RNA in aqueous two-phase systems by the addition of neutral salt.
by: Luechau, Frank, et al.
Published: (2009)

A descriptive model and methods for up-scaled process routes for interfacial partition of bioparticles in aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2010)

Two-step process for initial capture of plasmid DNA and partial removal of RNA using aqueous two-phase systems.
by: Luechau, Frank, et al.
Published: (2010)

Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
by: Luechau, Frank, et al.
Published: (2009)

Physical characterisations of a single-stage Kühni-type aqueous two-phase extraction column
by: Luechau, Frank, et al.
Published: (2010)

Interfacial partitioning behaviour of bovine serum albumin in polymer-salt aqueous two-phase system
by: Chow, Yin Hui, et al.
Published: (2013)

Redefinition of working aqueous two-phase systems: a generic description for prediction of the effective phase chemical composition for process control and biorecovery
by: Selvakumar, Pitchaivelu, et al.
Published: (2010)

Characterization of partitioning behaviors of immunoglobulin G in polymer-salt aqueous two-phase systems
by: Yin, Hui Chow, et al.
Published: (2016)

Partitioning behaviours and selective recovery of therapeutic protein in aqueous two-phase system
by: Chow, Yin Hui
Published: (2016)

Separation of cyclodextrins (CDs) using aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2012)

AQUEOUS TWO-PHASE POLYMER MIXTURE SYSTEM AS PROTEIN XTRACTION TECHNIQUE. MODELLING OF PROTEIN PARTITIONING BEHAVIOR
by: Ahmad, Murni M
Published: (2007)

Integration of mechanical cell disruption and fluidised bed recovery of G3PDH from unclarified disrupted yeast: a comparative study of the performance of unshielded and polymer shielded dye-ligand chromatography systems
by: Ling, Tau Chuan, et al.
Published: (2005)

Separation of single-walled carbon nanotubes using aqueous two-phase system
by: Tang, Malcolm S. Y., et al.
Published: (2014)

Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system
by: M., Perumalsamy, et al.
Published: (2007)

Effect of polymer shielding on elution of G3PDH bound to dye-ligand adsorbent
by: Ling, Tau Chuan, et al.
Published: (2006)

The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract
by: Ling, Tau Chuan, et al.
Published: (2005)

Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
by: Ng, Hui Suan, et al.
Published: (2011)

Method for preparing plasmid DNA
by: Mohamed, Farahidah, et al.
Published: (2015)

Primary recovery of lipase derived from Burkholderia sp. ST8 with aqueous micellar two-phase system.
by: Chien, Wei Ooi, et al.
Published: (2011)

The Sykes-Picot agreements – phase two of the partition process
by: Guediri, Kaoutar
Published: (2016)

Extractive bioconversion of cyclodextrins by Bacillus cereus cyclodextrin glycosyltransferase in aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2013)

Extractive fermentation using aqueous two-phase systems for integrated production and purification of extracellular lipase derived from Burkholderia pseudomallei
by: Ooi, Chien Wei, et al.
Published: (2011)

Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH
by: Settu, sarananan, et al.
Published: (2007)

Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems
by: Ooi, Chien Wei, et al.
Published: (2009)

Recovery of Bacillus cereus cyclodextrin glycosyltransferase using ionic liquid-based aqueous two-phase system
by: Ng, Hui Suan, et al.
Published: (2014)

Direct purification of Burkholderia Pseudomallei lipase from fermentation broth using aqueous two-phase systems.
by: Ooi, Chien Wei, et al.
Published: (2009)

Removal of endotoxins from plasmid DNA: Analysis of aggregative interaction of mobile divalent metal cations with endotoxins and plasmid DNA
by: Ongkudon, C., et al.
Published: (2012)

Modeling of crude oil biodegradation using two phase partitioning bioreactor
by: Ahmadun, Fakhru'l-Razi, et al.
Published: (2014)

Effect of Pluronic F-68, 5% CO2 atmosphere, HEPES, and antibiotic-antimycotic on suspension adapted 293 cells
by: Negrete, Alejandro, et al.
Published: (2008)

Production of adenoviral vectors in 293 cells: a case study of the adaptation of attached cells to grow in suspension
by: Negrete, Alejandro, et al.
Published: (2008)

Direct recovery of lipase derived from Burkholderia cepacia in recycling aqueous two-phase flotation
by: Show, Pau Loke, et al.
Published: (2011)

Purification of the recombinant enhanced green fluorescent protein from Escherichia coli using alcohol + salt aqueous two-phase systems
by: Sewn, Cen Lo, et al.
Published: (2018)

Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems
by: Lin, Yu Kiat, et al.
Published: (2013)

Design of Aqueous Two-Phase Protein Separation Systems
by: Ahmad, Murni M
Published: (2005)

Effects of kinetics and mechanisms of crystal growth on ion-partitioning in solid solution-aqueous solution (SS-AS) systems
by: Putnis, Andrew
Published: (2010)

Procedure to develop binodal curve and phase diagram for aqueous two-phase system
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2018)

Extractive bioconversion of polycaprolactone by lipase in aqueous two-phase system / Chew Pey Ling
by: Chew, Pey Ling
Published: (2015)

Production of y-cyclodextrin by Bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system
by: Lin, Yu Kiat, et al.
Published: (2016)

Thermodynamic Modeling and Simulation of Aqueous Two-Phase Bioseparation Systems
by: Ahmad, Murni M
Published: (2003)