Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation.
Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids—oxoputerine and liriodenine—were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
MDPI
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/15237/ http://psasir.upm.edu.my/id/eprint/15237/1/Inhibitory%20effects%20of%20phylligenin%20and%20quebrachitol%20isolated%20from%20Mitrephora%20vulpina%20on%20platelet%20activating%20factor%20receptor%20binding%20and%20platelet%20agregation.pdf |
| _version_ | 1848842620327952384 |
|---|---|
| author | Moharam, Bushra Abdulkarim Jantan, Ibrahim Jalil, Juriati Shaari, Khozirah |
| author_facet | Moharam, Bushra Abdulkarim Jantan, Ibrahim Jalil, Juriati Shaari, Khozirah |
| author_sort | Moharam, Bushra Abdulkarim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids—oxoputerine and liriodenine—were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 µM, respectively. The IC50 value of phylligenin was comparable to that of cedrol (10.2 µM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP.
|
| first_indexed | 2025-11-15T08:02:02Z |
| format | Article |
| id | upm-15237 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:02:02Z |
| publishDate | 2010 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-152372015-10-21T03:53:10Z http://psasir.upm.edu.my/id/eprint/15237/ Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. Moharam, Bushra Abdulkarim Jantan, Ibrahim Jalil, Juriati Shaari, Khozirah Phylligenine, together with quebrachitol, stigmasterol and two aporphine alkaloids—oxoputerine and liriodenine—were isolated from the twigs of Mitrephora vulpina C.E.C. Fisch. They were evaluated for their ability to inhibit platelet activating factor (PAF) receptor binding to rabbit platelets using 3H-PAF as a ligand and their antiplatelet aggregation effect in human whole blood induced by arachidonic acid (AA), collagen and adenosine diphosphate (ADP). Of all the compounds tested, phylligenin and quebrachitol exhibited potent and concentration-dependent inhibitory effects on PAF receptor binding, with IC50 values of 13.1 and 42.2 µM, respectively. The IC50 value of phylligenin was comparable to that of cedrol (10.2 µM), a potent PAF antagonist. Phylligenin also showed strong dose-dependent inhibitory activity on platelet aggregation induced by AA and ADP. MDPI 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15237/1/Inhibitory%20effects%20of%20phylligenin%20and%20quebrachitol%20isolated%20from%20Mitrephora%20vulpina%20on%20platelet%20activating%20factor%20receptor%20binding%20and%20platelet%20agregation.pdf Moharam, Bushra Abdulkarim and Jantan, Ibrahim and Jalil, Juriati and Shaari, Khozirah (2010) Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. Molecules, 15 (11). pp. 7840-7848. ISSN 1420-3049 10.3390/molecules15117840 English |
| spellingShingle | Moharam, Bushra Abdulkarim Jantan, Ibrahim Jalil, Juriati Shaari, Khozirah Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title | Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title_full | Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title_fullStr | Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title_full_unstemmed | Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title_short | Inhibitory effects of phylligenin and quebrachitol isolated from Mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| title_sort | inhibitory effects of phylligenin and quebrachitol isolated from mitrephora vulpina on platelet activating factor receptor binding and platelet agregation. |
| url | http://psasir.upm.edu.my/id/eprint/15237/ http://psasir.upm.edu.my/id/eprint/15237/ http://psasir.upm.edu.my/id/eprint/15237/1/Inhibitory%20effects%20of%20phylligenin%20and%20quebrachitol%20isolated%20from%20Mitrephora%20vulpina%20on%20platelet%20activating%20factor%20receptor%20binding%20and%20platelet%20agregation.pdf |